Effects of GDF11 on apoptosis of human papillary thyroid cancer K1 cells under high glucose conditions
YOU Yan1,2, TIAN Xiaoyu2,3, ZENG Lang1, DANG Ruijie2,3, ZENG Bangbin1, ZHU Biao3, LI Yunxia3
1. Department of Otolaryngology, Head and Neck Surgery, Guang'an People's Hospital, Guang'an 638000, China; 2. Institute of Orthopedics, PLA General Hospital, Beijing 100853,China; 3. Department of Stomatology, the First Medical Center, PLA General Hospital, Beijing 100853,China
Abstract：Objective To investigate the effects of GDF11 on apoptosis of human papillary thyroid cancer K1 cells under high glucose conditions. Methods Human papillary thyroid cancer K1 cells from the vehicle, HG (high glucose), and GDF11 (HG plus GDF11 administration) groups were cultured for 3 days. Flow cytometry was used to evaluate the apoptotic rate, while Western blot was used to investigate protein levels of Bax and Bcl-2. The expression levels of Nrf2 were determined via qRT-PCR while ROS levels in K1 cells were detected by ROS kit. Results Compared with the vehicle group, the apoptotic rate of HG was decreased, Bcl-2/Bax was increased, expression levels of Nrf2 were up-regulated, and ROS levels were significantly decreased. GDF11 administration increased K1 apoptosis, reduced Bcl-2/Bax protein levels, down-regulated Nrf2 expression levels, and increased ROS levels. Conclusions GDF11 may promote K1 cell apoptosis under high glucose conditions by activating intracellular oxidative stress.
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2021, Vol. 32 
