Mechanism of apoptosis of colon cancer cells induced by resveratrol through MYD88 / TLR4 / NF-KB signaling pathway
QIAO Lu1, YANG Yan2, TANG Daishi3, DU Ting3, GU Yong3
1. Department of Gastroenterology, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004,China; 2. Digestive System Department, the First Affiliated Hospital of AFMU, Xi'an 710032,China; 3. Digestive System Department, Shaanxi Provincial Crops Hospital of Chinese People's Armed Police Force, Xi'an 710054, China
Abstract:Objective To investigate the mechanism of apoptosis of colon cancer cells induced by resveratrol through MyD88/TLR4/ NF-KB signaling pathway.Methods With different doses of resveratrol for 24 h, TUNEL assay was used to detect the apoptosis rate of colon cancer cells and Western blot and RT-PCR were employed to test the expression of key signal molecules in MyD88/TLR4/NF-kB signaling pathway.Results The apoptosis rates of HT-29, SW480 and LoVo significantly increased after treatment. The protein and mRNA levels of Bax and Cleaved Caspase 3 significantly increased and Bcl-2 was downregulated. The expression of VEGF protein in cell supernatant decreased significantly. MyD88, TLR4 and NF-KB were significantly down regulated (P<0.05).Conclusions Resveratrol may promote the apoptosis of colon cancer cells through MyD88/TLR4/ NF-KB signaling pathway to inhibit the development of tumor.
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