Effect of polymyxin B on expression of LPS in peripheral blood of rats after craniocerebral trauma
ZHOU Xiaopin1, WANG Yanting2, LIU Bin1
1. Department of Infection, Characteristic Medical Center of Chinese People's Armed Police Force, Tianjin 300162, China; 2. Department of Neurosurgery, the 960th Hospital of PLA Joint Logistics Support Force, Jinan 250000, China
Abstract：Objective To explore the effect of polymycin B (PMB) on the expression of lipopolyssacharide (LPS)in peripheral blood of rats after craniocerebral trauma. Methods Fifty healthy 7-week-old adult male SD rats were randomly divided into 5 groups: normal group, sham group, traumatic brain injury group (TBI), traumatic brain injury+Normal saline group (TBI+NS group), traumatic brain injury + polymycin group (TBI+PMB group), with 10 rats in each group. Electronic cortical injury impingement instrument (eCCI) was used to construct models. The neurological deficit scores (NSS) of the rats in the first 3 groups were calculated at 24 h and 48 h before and after modeling. At 48h after modeling, the rats in the first three groups were given FD4 gavage, and the concentration of FD4 in the cycle was detected to indirectly reflect changes in intestinal permeability. The rats in TBI+PMB group were intraperitoneally injected with PMB solution (2.5 μg/g) through 1 ml syringe, and those in TBI+ NS group were given equal volume of normal saline. At the time points of 0 d, 1 d, 3 d, 5 d and 7 d, the concentration of LPS in peripheral blood was detected by limulus lysate end-point colorimetric method. Paraffin-embedded HE staining and inflammation scores were used to evaluate the inflammatory response of brain tissue at 7 d. Results Compared with the sham group[(1850±80) ng/ml], the concentration of FD4 in the normal group and the TBI group increased [(2743±279) ng/ml vs. (4228±203) ng/ml](P<0.05); compared with the sham group, LPS concentration in TBI group significantly increased at day 3 [(0.94±0.02) EU/ml vs. (0.45±0.06) EU/ml], and there was no significant difference at other time points. Compared with TBI+NS group, LPS concentration in TBI+PMB group significantly decreased at day 3 after operation [(0.56±0.04) EU/ml vs. (0.94±0.03) EU/ml, P<0.001];inflammation scores in TBI+PMB group were significantly lower than those in TBI group and TBI+NS group. Conclusions The acute TBI can cause intestinal flora shifting and transient increase of LPS level in peripheral blood of rats. PMB can reduce the early LPS concentration after TBI and reduce the inflammatory response in brain tissues after acute TBI.
周小嫔, 王衍廷, 刘斌. 多黏菌素B干预颅脑创伤后大鼠外周血LPS表达的变化[J]. 武警医学, 2022, 33(10): 829-833.
ZHOU Xiaopin, WANG Yanting, LIU Bin. Effect of polymyxin B on expression of LPS in peripheral blood of rats after craniocerebral trauma. Med. J. Chin. Peop. Armed Poli. Forc., 2022, 33(10): 829-833.
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