Abstract：Objective To study the therapeutic effect of salvia miltiorrhiza on oral submucosal fibrosis in rats. Methods After the establishment of the SD rat OSF model using arecoline smear method, the rats were divided into 6 experimental groups and 1 control group. The experimental group received 5 mg/ml tanshinone ⅡA, tanshinone ⅡB, cryptotanshinone, isocryptotanshinone, salvianolic acid A, and salvianolic acid B, while the control group were treated with physiological saline. After 8 weeks of treatment, the local mucosal lesion area was measured and the local buccal mucosa was stained with HE to observe the pathological changes. The expressions of type Ⅲ collagen, TGF-β1 and IFN-γ were detected. Results The area of oral submucosal fibrosis in the tanshinone ⅡA, cryptotanshinone and isocryptotanshinone groups decareased by 2.72, 1.95, and 1.58 cm2, respectively, and the cure rate was 60%-80%, which was statistically significant compared to the control group.The type Ⅲ collagen in the tanshinone ⅡA, cryptotanshinone, and iso cryptotanshinone groups decreased by 0.06, 0.04, 0.03,TGF-β1 decreased by 0.08, 0.04, 0.05, and IFN-γ increased by 0.06, 0.03, 0.03, with statistically significant differences compared to the control group (P＜0.05).There was no statistically significant difference between the tanshinone ⅡB, salvianolic acid A, and salvianolic acid B groups and the control group. Conclusions Tanshinone ⅡA, Cryptotanshinone and Isocryptotanshinone have therapeutic effects on OSF in rats, while Tanshinone ⅡB, salvianolic acid A and salvianolic acid B do not have therapeutic effects.
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