Effects of urolithin A on browning of white adipose in aged mice fed on a high-fat diet
LIU Xingyu1, LI Hui2, LI Jing3, WANG Ying4, ZHAO Lisheng4, FENG Chunzhen4
1. the First Healthcare Department, the Second Medical Center of PLA General Hospital, Beijing 100048, China; 2. Department of Geriatrics, Mudanjiang Hospital of Beidahuang Group, Jixi 158300, China; 3. Department of Stomatology, Beijing Friendship Hospital of Capital Medical University, Beijing 100050, China; 4. Department of Stomatology, the First Medical Center of PLA General Hospital, Beijing 100853, China
Abstract：Objective To investigate the effects of urolithin A on browning of white adipose in aged mice fed on a high-fat diet. Methods Eighteen aged mice were randomly divided into control group, high-fat diet (HFD) group and urolithin A administration group (UA), with 6 mice in each group. UA group was given urolithin A (Tween-80 aid solution), control group and HFD group were given the same dose of Tween-80. After the experiment, the body weight and heat production were detected, the morphology of adipocytes was observed by HE staining. qRT-PCR and Western blot were used to investigate mRNA and protein levels of uncoupling protein 1 (UCP1), peroxisome proliferator-activated receptor γ (PPARγ) and peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α). Results Compared with the control group, the body weight of HFD group significantly increased, the heat production of the daytime[(0.45±0.03) kcal/h vs. (0.62±0.05) kcal/h] and nighttime[(0.49±0.05) kcal/h vs. (0.73±0.06) kcal/h] significantly decreased(P<0.05), and the cell size of white adipose tissue increased. Moreover, UCP1, PPARγ and PGC-1α expressions decreased (P<0.05). However, urolithin A intervention reduced the body weight of high-fat diet mice, increased daytime heat production [(0.57±0.03) kcal/h vs. (0.45±0.03) kcal/h] and nighttime heat production [(0.69±0.07) kcal/h vs. (0.49±0.05) kcal/h], and promoted the transcriptional expression of UCP1, PPARγ and PGC-1α expressions (P<0.05). Conclusions Urolithin A can promote the browning of white adipose tissue in aged mice fed on a high-fat diet.
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LIU Xingyu, LI Hui, LI Jing, WANG Ying, ZHAO Lisheng, FENG Chunzhen. Effects of urolithin A on browning of white adipose in aged mice fed on a high-fat diet. Med. J. Chin. Peop. Armed Poli. Forc., 2023, 34(11): 926-929.
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