Analysis of active components in serum of modified prescription of Banxia Xiexin decoction and its antibacterial effect in vitro
SHAO Juan1, LIANG Yu1, ZHENG Yating1, CAO Youjun2, CHEN Jie3, Qian Yunda3, KONG Guimei4
1. Yangzhou Branch of Jiangsu Corps of Chinese People's Armed Police Force,Yangzhou 225007,China; 2. Department of Radiation Oncology,Mingji Hospital Affiliated to Nanjing Medical University,Nanjing 210019,China; 3. Department of Gastroenterology,Jiaxing Hospital of Traditional Chinese Medicine Affiliated to Zhejiang University of Traditional Chinese Medicine,Jiaxing 314000,China; 4. Jiangsu Provincial Key Laboratory of Zoonosis,Yangzhou 225009,China
Abstract：Objective To analyze the main active components in the serum of Banxia Xiexin decoction were analyzed,and its antibacterial effect in vitro. Methods After decocting and concentrating Banxia Xiexin decoction,the drug-containing serum of Banxia Xiexin decoction was prepared by intragastric administration to rats. The main drug active components in the serum were determined by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS-MS). According to the results of HPLC-MS-MS results,the inhibitory effects of main active components and drug-containing serum of Banxia Xiexin decoction on Helicobacter pylori and other bacteria were detected by microbroth dilution method in vitro. Results The test results showed that the contents of the main active components in the drug-containing serum, from high to low, were quercetin, isorhamnetin, baicalin, succinic acid, and codonopsis glycoside. The results of the in vitro antibacterial experiments showed that minimum inhibitory concentration (MIC) of codonopsis pilosula for inhibiting bacteria （staphylococcus aureus, staphylococcus albicans, escherichia coli, salmonella, proteus, helicobacter pylori） was 100μg/ml.,the MIC of isorhamulin was 400 μg/ml for helicobacter pylori,and the remaining bacteria were 200 μg/ml. The MIC of baicalin in helicobacter pylori was 400 μg/ml,and others were 200 μg/ml. The MIC of quercetin was 200 μg/ml. The MIC of drug containing serum was in a volume ratio of 1：4 （staphylococcus aureus,escherichia coli and proteus） and 1：1 （salmonella and helicobacter pylori）. Succinic acid did not inhibit the growth of these bacteria. Conclusion The main active components of the drug-containing serum in the modified formula of Banxia Xiexin decoction, namely codonopsis glycoside,baicalin,quercetin and isorhamnine,have the effect of inhibiting helicobacter pylori and other bacteria, clarifying the main reason the main reasons for the antibacterial and anti-inflammatory effects of the modified formula of Banxia Xiexin decoction.
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SHAO Juan, LIANG Yu, ZHENG Yating, CAO Youjun, CHEN Jie, Qian Yunda, KONG Guimei. Analysis of active components in serum of modified prescription of Banxia Xiexin decoction and its antibacterial effect in vitro. Med. J. Chin. Peop. Armed Poli. Forc., 2025, 36(5): 416-420.
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