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| Expression and significance of gene mRNA of urokinase type plasminogen activator and its inhibitor 1 type in muscle of exprimental autoimmune myositis guinea pigs |
| DUAN Feng |
| Department of Neurology, Navy General Hospital, Beijing 100048, China |
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Abstract Objective To study the expression and significance of gene mRNA of urokinase type plasminogen activator and its inhibitor 1type(PAI-1)in the muscle of exprimental autoimmune myositis (EAM) guinea pigs. Methods The EAM model was established by repeatedly immunizating guinea pigs with rabbit skeletal muscle myosin plus Freund's complete adjuvant and giving these pigs a celiac injection of pertussis toxin. The changes in clinical symptoms, serum creatine kinase and pathology were observed. The expression level of uPA and PAI-1 in the skeletal muscle was detected by RT-PCR and compared between EAM group ( n =11) and normal group ( n =10). Results (1) The level of expression of the uPA and PAI-1 mRNA in the muscle increased significantly in EAM group, compared with normal group（ P <0.01). (2)There was no difference in the ratio between expression of the uPA and PAI-1 mRNA in the muscle tissues of EAM group and normal group ( P <0.01). Conclusions uPA and PAI-1 may contribute to the pathogenesis of EAM.
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Received: 09 February 2012
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