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| The role of CML28 in regulation of proliferation and apoptosis of K562 cells |
| BAI Xueling1,2,MAO Xia1,ZHANG Bing1,CAO Fengbin2,and ZHANG Donghua1. |
| 1. Department of Hematology, Tongji Hospital affiliated to Tongji Medical College, Huazhong University of Science ＆ Technology. Wuhan 430030，China; 2.Medical Team of Luoyang Military Detachment, Henan Provincial Corps, Chinese Armed Police Forces, Luoyang 471000，China |
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Abstract Objective To explore the role of CML28 in regulation of proliferation and apoptosis of K562 cells. Methods K562 cells were cultured in vitro and electrotransfected with Si-hCML28. K562 cells which had been transfected were collected after 48 hours. Total mRNA was extracted from K562 cells using Trizol reagent and the first cDNA was synthesized using the reverse transcription kit.The silence effect was detected by RT-PCR and Real time PCR. The ratio of apoptosis and proliferation of K562 cells was detected by Flow Cytometry (FCM)after CML28 was down-regulated. Data were analyzed by SPSS17.0 statistics software and P <0.05 was considered statistically significant. Results (1) There was a high level of CML28 in K562 cells; (2)The mRNA expression of CML28 was 0.524 ± 0.044 after interference, lower than that of negative control. There was statistically significant difference；(3) The apoptosis ratio was (22.45±3.54)% in the group of Si-hCML28, higher than that of negative control. Conclusions CML28 is highly expressed in K562 cells. The mRNA expression of CML28 can be effectively down-regulated by Si-hCML28. In addition, it can significantly inhibit K562 cell proliferation and induce apoptosis of K562 cells, suggesting that it plays an important role in regulating the apoptosis and proliferation of K562 cells.
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Received: 15 February 2012
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| ［1］ |
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2012, Vol. 23 
