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| Determination of three flavonoids in Apocynum venetum by high-performance capillary electrophoresis |
| LI Fang1,MA Xiaoli2,SUN Lian3,and MENG Lei2. 1. Department of Pharmacy,Xinjiang Production and Construction Corps Hospital of Chinese People’s Armed Police Forces,Urumqi 830063,China; 2. Analytical & Testing Center,3. College of Pharmacy,Xinjiang Medical University,Urumqi 830011,China |
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Abstract Objective To establish a high-performance capillary electrophoretic (HPCE) method for simultaneous determination of contents of rutin, isoquercitrin，and quercetin in Apocynum venetum. Methods A bare fused-silica capillary (58.5 cm×75 μm, effective length of 48.5 cm)was used. The buffer was composed of 50 mmol/L Na2B4O7 (pH 9.60). The running voltage was set at 20 kV with polarity from anode (inlet) to cathode (outlet). The separation time was 20 min and temperature was set at 25 ℃. Online detection was performed at 350 nm (PDA). Results The three types of flavonoids were well separated in 25 minutes and linear relationship in the peak area was good. The linear range was 4.4-44, 2.8-28.2, and 3.8-38 μg/ml, respectively. The correlation coefficient was 0.9968, 0.9995, and 0.9991, respectively. The average recovery was 97.3%, 98.4%, and 98.3%, respectively. Conclusions This method is simple, rapid, accurate, and reliable, which can be used for content determination of flavonoids in Apocynum leaves.
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Received: 17 March 2012
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张素琼，燕 虹，李青山. 罗布麻叶有效部位降血脂及抗动脉粥样硬化的研究［J］.中西医结合心脑血管病杂志,2007,5(9):831-832.［2］ 钱曾年,顾振伦.大花罗布麻叶的药理学研究Ⅳ：对心血管系统的影响［J］.中成药,1991，13(7):27-29.［3］ 郑永红，韦晓瑜，龙继红.罗布麻叶中黄酮类成分研究进展［J］.今日药学，2010,20（8）:5-7.［4］ 范维刚.反相高效液相色谱法罗布麻叶中槲皮素和山萘酚［J］.分析试验室,2008，27(8)：52-54.［5］ 韩利文, 侯晋军, 李云兰,等.高效液相色谱法比较不同种属和产地罗布麻叶中金丝桃苷的含量［J］.中国现代应用药学,2006，23（5）：392-394.［6］ 张群林，吴 亮，言安定,等.HPLC同时测定罗布麻叶药材及其提取物中6种黄酮的含量［J］.中国中药杂志，2011，3（5）：589-592.
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张素琼，燕 虹，李青山. 罗布麻叶有效部位降血脂及抗动脉粥样硬化的研究［J］.中西医结合心脑血管病杂志,2007,5(9):831-832.［2］ 钱曾年,顾振伦.大花罗布麻叶的药理学研究Ⅳ：对心血管系统的影响［J］.中成药,1991，13(7):27-29.［3］ 郑永红，韦晓瑜，龙继红.罗布麻叶中黄酮类成分研究进展［J］.今日药学，2010,20（8）:5-7.［4］ 范维刚.反相高效液相色谱法罗布麻叶中槲皮素和山萘酚［J］.分析试验室,2008，27(8)：52-54.［5］ 韩利文, 侯晋军, 李云兰,等.高效液相色谱法比较不同种属和产地罗布麻叶中金丝桃苷的含量［J］.中国现代应用药学,2006，23（5）：392-394.［6］ 张群林，吴 亮，言安定,等.HPLC同时测定罗布麻叶药材及其提取物中6种黄酮的含量［J］.中国中药杂志，2011，3（5）：589-592.
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2012, Vol. 23 
