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| Fluorescence and chromogenic substrates rapid test for screening influenza A virus |
| YU Hongyu1,WANG Shuntao2,LIU Bin3,and LI Xiaofang3. |
| 1. Shenzhen Beike Cell Engineering Institute, Shenzhen 518057; 2. Biotherapeutic Center, 2nd Hospital of Beijing Armed Police Forces, Beijing 100037; 3. Key Laboratory of Theoretical Chemistry and Molecular Simulation of Ministry of Education, Hunan University of Science and Technology, Xiangtan 411201, China |
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Abstract Objective To evaluate the diagnostic efficiency of fluorescence and chromogenic substrates rapid tests in clinical screening of influenza A virus. Methods A total of 377 suspected flu patients’ nasopharynx secretions were examined by fluorescence and chromogenic substrates rapid tests. Then the clinical diagnostic value was evaluated by using the virus nucleic acid detection results as the standard. Results The sensitivities of two methods were 79.08% and 90.2%, and the specificities of them 91.96% and 95.09%, respectively. The difference between the two methods for influenza A virus detection was not statistically significant（χ2=1.25,P>0.05）. Conclusions Compared with PCR assays, the two methods for influenza A virus detection are both valuable in clinical application, but fluorescence substrate assay is more sensitive than chromogenic substrate assay; the former is suitable for clinical rapid diagnosis, and the latter is more simple in detecting the massive specimens or field test.
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Received: 27 April 2013
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| ［1］ |
李增德, 张伟龙, 许凌峰,等. 新甲型H1N1流感病毒分子生物学研究进展 ［J］. 武警医学, 2010, 21(3):262-264.［2］ Hui Donga, Zhang Yanjun, Hui Xiong, et al. Detection of human novel influenza A (H1N1) viruses using multi-fluorescent real time RT-PCR ［J］. Virus Research, 2010, 147(1): 85-90.［3］ 孙珊珊, 沈国顺, 田明尧, 等. 甲型H1N1与季节性H1N1流感病毒检测基因芯片的制备与初步应用 ［J］. 中国兽医学报, 2010, 30(5): 634-638.［4］ Colman P M. Influenza virus neuraminidase: structure, antibodies and inhibitors ［J］. Protein Sci, 1994, 3: 1687-1696.
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李增德, 张伟龙, 许凌峰,等. 新甲型H1N1流感病毒分子生物学研究进展 ［J］. 武警医学, 2010, 21(3):262-264.［2］ Hui Donga, Zhang Yanjun, Hui Xiong, et al. Detection of human novel influenza A (H1N1) viruses using multi-fluorescent real time RT-PCR ［J］. Virus Research, 2010, 147(1): 85-90.［3］ 孙珊珊, 沈国顺, 田明尧, 等. 甲型H1N1与季节性H1N1流感病毒检测基因芯片的制备与初步应用 ［J］. 中国兽医学报, 2010, 30(5): 634-638.［4］ Colman P M. Influenza virus neuraminidase: structure, antibodies and inhibitors ［J］. Protein Sci, 1994, 3: 1687-1696.
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2013, Vol. 24 
