血清MicroRNA-122作为脓毒症诊断特异性标志物的研究

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Abstract Objective To study the dynamic changes in serum miRNA-122 levels during sepsis and the correlation between serum miRNA-122 and sepsis severity. Methods Fifty-two sepsis patients (including 23 non-severe septic patients and 29 severe septic patients)and 23 healthy controls were recruited in the study. The expression levels of serum miRNA-122 were quantified by quantitative reverse transcriptase polymerase chain reaction assays (qRT-PCR).Levels of serum miRNA-122 were detected on the 1 st, 3 rd, 5 th, 7 th, 10 th and 14 th day after their admissions in intensive care unit. All the patients clinical information was also recorded. Results After comparison, levels of serum miRNA-122 in sepsis patients on every observation time point were all significantly lower than those in healthy controls (P＜0.01). The receiver operating characteristic curve was obtained and the area under curve was calculated on the levels of serum miRNA-122 on the first day of their admissions. When the cut-off point was set at 0.710, miRNA-122 had a specificity of 95.7% and a sensitivity of 53.8%. The expression levels of serum miRNA-122 in severe sepsis group were significantly higher than in sepsis group on each observation time point (P＜0.01). Conclusions Serum miRNA-122 can used as a specific biomarker for diagnosis of sepsis. And miRNA-122 might be correlated with the organ injuries of septic patients.

Key words： sepsis microRNA-122 microRNA diagnosis

Received: 27 November 2012

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	Articles by authors
	DENG Jie
	WANG Huijuan
	SU Longxiang
	ZHANG Xin
	XIAO Kun
	JIA Yanhong
	and XIE Lixin

Cite this article:

DENG Jie,WANG Huijuan,SU Longxiang等. Serum microRNA-122 as a specific marker of sepsis diagnosis[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2013, 24(5): 383-386.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2013/V24/I5/383

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