丙型肝炎病毒核酸定量和抗-HCV血清学分析的诊断价值

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Abstract Objective To study the application value of hepatitis C virus RNA detection (PCR-fluorescent probe) and hepatitis C virus antibody detection (ELISA, anti-HCV) in the diagnosis of hepatitis C. Methods The HCV-RNA and anti-HCV tests of 265 suspected HCV patients were reviewed, statistics of anti-HCV and HCV-RNA detection results and ALT level in different ages and gender was collected and analysized. Results ①Anti-HCV and HCV-RNA positive rates in men were higher than those in women, but there was no statistically significant difference (P>0.05).② Anti-HCV and HCV-RNA positive rates in＞40 years old patients were obviously higher than those in ≤40 years old patients ,with statistically significant difference (P<0.05); ALT abnormal rates also had significant difference between＞40 and ≤40 years old patients (P<0.05).③ ALT abnormal rate increased with increasing HCV-RNA positive rate and have positive correlation with HCV-RNA content(r=0.999,P<0.05). But the ALT levels did not correlate with HCV-RNA content (r=0.800,P>0.05). ④The abnormal rate of Anti-HCV was closely correlated with HCV-RNA (r=0.320); Anti-HCV, HCV-RNA and ALT had no statistically significant difference (P>0.05). Conclusions ①Anti-HCV and HCV-RNA detection have no relation with gender, but they have certain relevance with age. ②ELISA for detecting anti-HCV is still first choice in HCV. Combined use of HCV-RNA,anti-HCV and ALT detection, will be the most reliable and valuable method in diagnosis of HCV.

Key words： HCV RNA quantification HCV antibody diagnosis

Received: 02 July 2013

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	Articles by authors
	LIU Xianhua
	BAI Xiaodong
	LIU Yuanming
	DUAN Cuijuan
	RONG Ran
	and LIU Weiwei.

Cite this article:

LIU Xianhua,BAI Xiaodong,LIU Yuanming等. Retrospective analysis of Antibody of HCV and quantification of HCV RNA in diagnosis of hepatitis C[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2013, 24(6): 488-490.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2013/V24/I6/488

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