重楼皂苷促成骨样细胞MC3T3-E1增殖作用及其机制

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Abstract Objective To investigate the effect of polyphyllin on the proliferation of osteoblast-like cells MC3T3-E1 and possible mechanisms. Methods The osteoblast-like cells MC3T3-E1, were administered with different concentrations of polyphyllin (3, 10, 30, and 100 μg/ml). Then, MTT colorimetric test, EdU kit, and alkaline phosphatase (ALP) activity detection kit were used to observe the proliferation of MC3T3-E1. Western blot analysis was employed to observe the expression of β-catenin and bone morphogenetic protein -2 (BMP-2) in MC3T3-E1 cells treated with 30, and 100 μg/ml of polyphyllin. Results Polyphyllin could effectively promote proliferation of MC3T3-E1 cells and upregulate the expression of β-catenin and BMP-2 expression. Conclusions Polyphyllin may promote MC3T3-E1 cell proliferation and differentiation via regulating β-catenin and BMP-2 signaling pathway.

Key words： polyphyllin osteoblast-like cells MC3T3-E1 proliferation bone morphogenetic protein 2 β-catenin

Received: 03 April 2014

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XU Xiao-Lian,LI Bo. Effect of polyphyllins on proliferation of osteoblast-like cells MC3T3-E1 and its possible mechanisms[J]. , 2014, 25(3): 275-277.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2014/V25/I3/275

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