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| Inhibition of miR21 inhibitor on A-549 cells in vitro |
| JIANG Xuedong,WANG Qiong,LIU Changhao,WANG Guozhu |
| Department of Thoracic Cardiovascular Surgery, Liaoning Provincial Corps Hospital, Chinese People’s Armed Police Forces, Shenyang 110034, China |
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Abstract Objective To study the inhibition of miR21 inhibitor on the invasion and anti-angiogenesis of A-549 cells. Methods MiR21 inhibitor was transfected into A-549, and the control group was set up. 24, 48, 72, 96 hours after transfection, the effect of growth suppressing was quantified by 3-2(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide(MTT) assay, anti-invasion was observed by transwell assay. Tube formation assay was used to detect the effects of miR21 inhibitor on human umbilical vein endothelial cells (HUVEC) angiogenesis. Results Twenty-four hours after transfection, the suppressing rate was different between the two groups; 48, 72, 96 h after transfection, the suppressing rates in inhibition group were higher than those in control group, the difference was statistically significant(P<0.05). By transwell assay, number of cells in inhibition group (11.60?5.32) was less than that in control group (107.60?3.21), the difference was statistically significant(P<0.01). Tubes in inhibition group (159.30?0.51) was less than that in control group (508.80?1.30), the difference was statistically significant(P<0.01). Conclusions miR21 inhibitor can inhibit the invasion of A-549 and angiogenesis of HUVEC. miR21 inhibitor can be a potential gene-therapy for gastric carcinoma.
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Received: 21 January 2014
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2014, Vol. 25 
