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| Seting-up a wide-field fluorescence endoscopic and its preliminary application |
| Qu Yawei, Xia Minxin, LIU Haifeng |
| Department of Gastroenterology, General Hospital of Chinese People’s Armed Police Forces, Beijing 100039, China |
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Abstract Objective To setup a new flexible wide-field epi-fluorescence endoscope system, then make a fluorescence imaging for gastric cancer xenograft model in nude mice by this system, so as to setup a new device and method for the diagnosis of gastric cancer molecular imaging techniques. Methods The widefield fluorescence endoscope system was assembledusing the rational optical path and optical components machining. Different concentrations of 2-DG-750 fluorescent probe was used to analyze the performance of the imaging system.By intravenous injection of 100 μl2-DG-750 fluorescent probe into the xenografted tumor model,in vivo fluorescence imaging wasconducted, and ROI pixel values and imaging map were analyzed. Results The ROI pixel values demonstrated that a sensitivity of 31.3 pmoles 2-DG-750 was achieved as well as a good linear response from 1nmole to zero fluorescent probe. Fluorescence imaging indicated that gastrointestinal tumor tissues have an eminent 2-DG-750 uptake yielding a relatively strong fluorescence signal. Conclusions The new flexible wide-field epi-fluorescence endoscope system not only has a good sensitivity, but also can do a dual white and fluorescence imaging, which makes a promise future that optical technique will be applied to the diagnosis of gastric cancer.
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Received: 06 December 2014
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| [1] |
Pillai R S, Lorenser D, Sampson D D, et al. Deep-tissue access with confocal fluorescence microendoscopy through hypodermic needles [J]. Opt Express, 2011, 19(8): 7213-7221.
|
| [2] |
Van Rijn J C, Reitsma J B, Stoker J, et al. Polyp miss rate determined by tandem colonoscopy: a systematic review[J]. Am J Gastroenterol, 2006, 101(2): 343-350.
|
| [3] |
Kee Wong Song L M, Adler D G, Chand B, et al. Chromoendoscopy [J]. Gastrointest Endosc, 2007, 66(4): 639-649.
|
| [4] |
Song L, Banerjee S, Desilets D, et al. Autofluorescence imaging [J]. Gastrointest Endosc, 2011, 73(4): 647-650.
|
| [5] |
Song L M, Adler D G, Conway J D, et al. Narrow band imaging and multiband imaging [J]. Gastrointest Endosc, 2008, 67(4): 581-589.
|
| [6] |
Soon L, Braet F, Condeelis J. Moving in the right direction — nanoimaging in cancer cell motility and metastasis[J].Microsc Res Tech,2007,70(3):252-257.
|
| [7] |
Deisseroth K, Feng G, Majewska A K, et al. Next-generation optical technologies for illuminating genetically targeted brain circuits [J]. J Neurosci, 2006, 26(41): 10380-10386.
|
| [8] |
Muguruma N, Ito S. Labeled anti-mucin antibody detectable by infrared-fluorescence endoscopy [J]. Cancer Biomarkers, 2008, 4(6): 321-328.
|
| [9] |
Choi Y, Kim K G, Kim J K, et al. A novel endoscopic fluorescent clip for the localization of gastrointestinal tumors [J]. Surg Endosc, 2011, 25(7): 2372-2377.
|
|
|
|
2015, Vol. 26 
