直线加速器X线照射对人肺成纤维细胞经典Wnt通路中关键信号蛋白的影响

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Abstract Objective To investigate the effect of irradiation with X-ray linear accelerator on the key signaling proteins of canonical Wnt pathway in human lung fibroblasts (HLFs). Methods HLFs were divided into irradiated group and normal group. R group was irradiated by linear accelerators X-ray(5 Gy). After 24 hours, the expression of the protein levels of β-catenin in nucleus was examined by Western blot, the mRNA level of the HLFs was examined by real time PCR,and the levels of WISP-1 protein in conditioned medium (CM) was examined by ELISA. Results Compared with the normal control group, the protein and gene expression level of beta-catenin increased in irradiated group HLFs,the same to the protein expression levels of WISP-1,which were statistically significant(P<0.05).Conclusion Irradiation with X-ray linear accelerator can induce the activation of canonical Wnt signaling pathway in HLFs and promote the formation of pulmonary fibrosis,which probably provide the new target of the treatment of radiation-induced lung injury.

Key words： radiation-induced lung injury Wnt signaling pathway lung fibroblasts pulmonary fibrosis linear accelerator

Received: 14 January 2015

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R818.02

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	ZHU Yan
	ZHANG Chunyang
	FENG Huasong
	CHEN Xuxin
	WANG Wei

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ZHU Yan,ZHANG Chunyang,FENG Huasong等. Effect of X-ray linear accelerator on the signaling proteins of canonical Wnt pathway in human lung fibroblasts[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2015, 26(8): 787-790.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2015/V26/I8/787

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