闭合性颅脑损伤大鼠血清和海马SAX2蛋白质谱的表达

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Abstract Objective To study the alteration of SAX2 protein expression pattern in rat serum and hippocampus after closed head injury and to find biomarkers of brain injury. Methods Male Sprague-Dawley rats were randomly divided into sham operation group and injury groups which were subjected to Marmarou’s brain injury and then subdivided into 4 h、8 h、12 h、24 h and 48 h groups according to the time elapsed after injury. The pathological study included H&E staining and toluidine blue staining. The change of protein expression pattern in serum and hippocampus were monitored with strong anion exchange chips (SAX2). Results (1)A total of 530 protein peaks were detected on SAX2 chips in serum,and a total of 147 protein peaks in hippocampus. (2) Among the total of protein peaks detected, the relative intensity of 45 protein peaks was shown to be differentially altered on SAX2 chips in serum of injury groups, compared with sham operation group(P＜0.05). There were 97 protein peaks in serum of injury groups, which could not be detected in sham operation group with SAX2 chips. (3) The relative intensity of 18 protein peaks was shown to be differentially altered on SAX2 chips in hippocampus of injury groups compared with sham operation group(P＜0.05). There were 40 protein peaks in hippocampus of injury groups which could not be detected in sham operation group with SAX2 chips. (4) On SAX2 chips, only one protein(3656Da) peak was detected not only in serum but also in hippocampus. Conclusions The alteration of SAX2 protein expression patterns in serum and hippocampus can be induced after brain injury. The SAX2 protein expression patterns of serum and hippocampus after brain injury are different, which suggests that part of protein in serum can be a production of blood cells gene expression induced by brain injury. The distinct proteins detected in serum and hippocampus includ the proteins which are significant, compared with sham operation group and those which are not detected in sham operation group. They can be biomarkers of brain injury.

Key words： brain injury rat protein chip serum hippocampus

Received: 01 July 2015

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	Articles by authors
	ZHAO Yangong
	SHU Qingming
	CHENG Rui
	BAI Xiao
	LUO Min
	and DUAN Yuanyuan

Cite this article:

ZHAO Yangong,SHU Qingming,CHENG Rui等. Alteration of SAX2 protein expression pattern in rat serum and hippocampus after closed head injury[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2015, 26(11): 1087-1090.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2015/V26/I11/1087

[1]	Marmarou A, Foda M A, vanden Brink W, et al. A new model of diffuse brain injury in rats[J].Neurosurg, 1994, 80:301-313.
[1]	Marmarou A, Foda M A, vanden Brink W, et al. A new model of diffuse brain injury in rats[J].Neurosurg, 1994, 80:301-313.
[2]	Lei P, Li Y, Chen X , et al. Microarray based analysis of microRNA expression in rat cerebral cortex after traumatic brain injury[J].Brain Res, 2009, 1284: 191-201.
[3]	舒清明, 李灵芝, 李志强, 等. 大鼠机械性脑损伤蛋白质表达谱的变化及意义[J].中国实验诊断学, 2009,13(11): 1534-1538.
[2]	Lei P, Li Y, Chen X , et al. Microarray based analysis of microRNA expression in rat cerebral cortex after traumatic brain injury[J].Brain Res, 2009, 1284: 191-201.
[3]	舒清明, 李灵芝, 李志强, 等. 大鼠机械性脑损伤蛋白质表达谱的变化及意义[J].中国实验诊断学, 2009,13(11): 1534-1538.
[4]	Grond-Ginsbach C, Hummel M, Wiest T, et al. Gene expression in human peripheral blood mononuclear cells upon acute ischemic stroke[J].J Neurol,2008,255:723.
[4]	Grond-Ginsbach C, Hummel M, Wiest T, et al. Gene expression in human peripheral blood mononuclear cells upon acute ischemic stroke[J].J Neurol,2008,255:723.
[5]	Tang Y, Nee A C, Lu A, et al. Blood genomic expression profile for neuronal injury[J]. Cereb Blood Flow Metab, 2003, 23:310-319.
[5]	Tang Y, Nee A C, Lu A, et al. Blood genomic expression profile for neuronal injury[J]. Cereb Blood Flow Metab, 2003, 23:310-319.
[6]	Wulfkuhle J D, Liotta L A, Petricoin E F. Proteomic applications for the early detection of cancer[J]. Nat Rev Cancer, 2003, 3:267-275.
[6]	Wulfkuhle J D, Liotta L A, Petricoin E F. Proteomic applications for the early detection of cancer[J]. Nat Rev Cancer, 2003, 3:267-275.