钛表面TiO<sub>2</sub>纳米管抗菌覆膜药物释放动力学表征及其抗菌活性体外研究

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Abstract Objective To determine the release kinetics of gentamicin from the TiO₂ nanotubes(TNS-G), and to detect its antibacterial activity in vitro. Methods TiO₂ nanotubes (NTS) were fabricated on the titanium surface by electrochemical anodization. These nanotubes were loaded with gentamicin using a lyophilization method and vacuum drying, and its pharmacokinetics was detected in phosphate buffer. Staphylococcus aureus was used to study the antibacterial properties of the NTS-G. There were three study groups: the commercially pure titanium(Cp-Ti)group, the NTS group, and the NTS-G group.We compared the antibacterial efficacy with each other by bacterial adhesion and colony counting in bacterial culture. Results Drug release of NTS-G could be divided into two parts: initial burst release and relatively slow release.The fast initial concentration was 51.50 μg/ml , and 73.13 μg/ml at the 6 th hours. Most of load gentamicin was released within approximately 9 hours.In the second phase, different drug release kinetics were observed, with very slow and linearly increasing cumulative release over a period of 90 h. The drug concentration was maintained at about 89.10 μg/ml. We found that NTS-G could significantly inhibit bacterial adhesion and bioflm formation, compared with Cp-Ti or NTS, (P<0.05). Conclusions Titanium dioxide nanotubes loaded with antibiotics can deliver a high concentration of antibiotics locally at a specifc site, thereby providing a new strategy to prevent implant-associated infections.

Key words： TiO₂ nanotubes gentamicin Drug-release kinetics Staphylococcus epidermidis antibacterial activity

Received: 22 April 2015

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	Articles by authors
	FENG Mingguang
	LIU Zhongtang
	WANG Liqiang
	XU Li
	YANG Haitao
	WANG Jian
	WANG Haiyang
	and QING Shixin

Cite this article:

FENG Mingguang,LIU Zhongtang,WANG Liqiang等. Drug-release kinetics characterization of gentamicin from TiO₂ nanotubes and its antibacterial activity in vitro[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2015, 26(11): 1129-1133.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2015/V26/I11/1129

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