|
|
|
| The distribution and the significance of laminin gene expression in hepatitis B virus-related hepatocellular carcinoma |
| WANG Peixiao1,2, HUANG Lihong3, ZHANG Li4, CHEN Hong2, and ZHANG Qing2 |
1.Institute of Organ Transplantation, 3.The graduate student affairs office,General Hospital of Chinese People’s Armed Police Force, Beijing100039,China;
2. Graduate Division of Xinxiang Medical University,Xinxiang 453003,China;
4.First Department of Surgery;
Shaanxi Provincial Corps Hospital of Chinese People’s Armed Police Force, Xi’an 710054, China |
|
|
|
|
Abstract Objective Investigated the expression of laminin gene in hepatitis B induced-hepatocellular carcinoma(HCC), both of the specificity of the expression. Method Objects were 215 patients of HBV induced-HCCwith cirrhosis in our hospital who had been in the liver transplantation operation from 2003/1 to 2013/5. We examined the expression of laminin in 215 cases of HCC and adjacent tissues by immunohistochemistry.As the normal controls were normal liver tissue and benign liver disease. Results Localization of laminin on hepatic sinusoidal endothelial cell (SEC) and neovascularization of the stromal tissue and membrane / plasma. The expression of the laminin postive on hepatic sinusoidal endothelial cell and neovascularization of the HCC tissues was 70.23％(151/215), with 57.20％(123/215) in cancer adjacent tissues,with 13.33％(3/30)in benign liver diseases tissues. The expression of laminin protein in tumour tissues was significantly higher than that in cancer adjacent tissues and benign liver diseases tissues (P=0.000). By clinicopathologic rank correlation analysis, the expression of laminin was associated with tumour microsatellite focal(P=0.026) ,tumor number(P=0.053) , vascular invasion(P=0.005)andTNM(P=0.029). Conclusion laminin expression at the protein levels was also observed in association with developing and metastasisof the hepatocellular carcinoma.
|
|
Received: 20 May 2015
|
|
|
|
|
|
| [7] |
Choi M R, An C H, Yoo N J. Laminin gene LAMB4 is somatically mutated and expressionally altered in gastric and colorectal cancers[J]. APMIS,2014,32:598-602.
|
| [1] |
Siege l R,Naishadham D,Jemal A,et al. Cancer statistics 2012[J].Ca Cancer J Clin,2012,62(1):10-29.
|
| [2] |
陈建国. 中国肝癌发病趋势和一级预防[J]. 临床肝胆病杂志, 2012,42(4):159-162.
|
| [8] |
Negishi Y, Hamano N, Tsunoda Y, et al. AG73-modified Bubble liposomes for targeted ultrasound imaging of tumor neovasculature[J]. Biomaterials, 2013, 34(2): 501-507.
|
| [3] |
Aldinger K A, Mosca S J, Tetreault M, et al. Mutations in LAMA1 cause cerebellar dysplasia and cysts with and without retinal dystrophy[J]. Am J Hum Genet, 2014, 95(2): 227-234.
|
| [9] |
Siqueira A S, Gama-de-Souza L N, Arnaud M V, et al. Laminin-derived peptide AG73 regulates migration, invasion, and protease activity of human oral squamous cell carcinoma cells through syndecan-1 and beta1 integrin[J]. Tumour Biol, 2010,31(1):46-58.
|
| [4] |
Fotouhi O, Adel F M, Kjellman M, et al. Global hypomethylation and promoter methylation in small intestinal neuroendocrine tumors: an in vivo and in vitro study[J]. Epigenetics,2014,9(7):987-997.
|
| [10] |
Freitas V M, Vilas-Boas V F, Pimenta D C, et al. SIKVAV, a laminin alpha1-derived peptide, interacts with integrins and increases protease activity of a human salivary gland adenoid cystic carcinoma cell line through the ERK 1/2 signaling pathway[J]. Am J Pathol, 2007, 171(1): 124-138.
|
| [5] |
Fidler I J.The seed and soil hypothesis at the millennium :Recentadvances in the pathogenesis of cancer metastasis[J]. Clin Exp Metastasis,1999,17:731.
|
| [11] |
梅文忠,李 承, 谭淑莲,等. 层黏连蛋白α1、α2、α3、α5亚基在人脑胶质瘤中的表达[J]. 福建医科大学学报,2008,42(4):306-309.
|
| [6] |
Ashktorab H, Rahi H, Wansley D, et al. Toward a comprehensive and systematic methylome signature in colorectal cancers[J]. Epigenetics,2013,8(8):807-815.
|
| [12] |
Chetty C, Khumalo T, Da CDB, et al. Anti-LRP/LR specific antibody IgG1-iS18 impedes adhesion and invasion of liver cancer cells[J]. PLoS One, 2014, 9(5): e96268.
|
| [7] |
Choi M R, An C H, Yoo N J. Laminin gene LAMB4 is somatically mutated and expressionally altered in gastric and colorectal cancers[J]. APMIS,2014,32:598-602.
|
| [13] |
Negishi Y, Omata D, Iijima H, et al. Preparation and characterization of laminin-derived peptide AG73-coated liposomes as a selective gene delivery tool[J]. Biol Pharm Bull,2010,33(10):1766-1769.
|
| [8] |
Negishi Y, Hamano N, Tsunoda Y, et al. AG73-modified Bubble liposomes for targeted ultrasound imaging of tumor neovasculature[J]. Biomaterials, 2013, 34(2): 501-507.
|
| [14] |
Negishi Y, Omata D, Iijima H, et al. Enhanced laminin-derived peptide AG73-mediated liposomal gene transfer by bubble liposomes and ultrasound[J]. Mol Pharm, 2010,7(1):217-226.
|
| [9] |
Siqueira A S, Gama-de-Souza L N, Arnaud M V, et al. Laminin-derived peptide AG73 regulates migration, invasion, and protease activity of human oral squamous cell carcinoma cells through syndecan-1 and beta1 integrin[J]. Tumour Biol, 2010,31(1):46-58.
|
| [15] |
Alminana N, Grau-Oliete M R, Reig F. In vitro effects of SIKVAV retro and retro-enantio analogues on tumor metastatic events[J]. Peptides, 2004,25(2):251-259.
|
| [10] |
Freitas V M, Vilas-Boas V F, Pimenta D C, et al. SIKVAV, a laminin alpha1-derived peptide, interacts with integrins and increases protease activity of a human salivary gland adenoid cystic carcinoma cell line through the ERK 1/2 signaling pathway[J]. Am J Pathol, 2007, 171(1): 124-138.
|
| [11] |
梅文忠,李 承, 谭淑莲,等. 层黏连蛋白α1、α2、α3、α5亚基在人脑胶质瘤中的表达[J]. 福建医科大学学报,2008,42(4):306-309.
|
| [12] |
Chetty C, Khumalo T, Da CDB, et al. Anti-LRP/LR specific antibody IgG1-iS18 impedes adhesion and invasion of liver cancer cells[J]. PLoS One, 2014, 9(5): e96268.
|
| [13] |
Negishi Y, Omata D, Iijima H, et al. Preparation and characterization of laminin-derived peptide AG73-coated liposomes as a selective gene delivery tool[J]. Biol Pharm Bull,2010,33(10):1766-1769.
|
| [14] |
Negishi Y, Omata D, Iijima H, et al. Enhanced laminin-derived peptide AG73-mediated liposomal gene transfer by bubble liposomes and ultrasound[J]. Mol Pharm, 2010,7(1):217-226.
|
| [15] |
Alminana N, Grau-Oliete M R, Reig F. In vitro effects of SIKVAV retro and retro-enantio analogues on tumor metastatic events[J]. Peptides, 2004,25(2):251-259.
|
|
|
|
2015, Vol. 26 
