丁酸钠对严重烫伤大鼠小肠黏膜血流量和微血管通透性的影响

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Abstract Objective To investigate the small intestine micro-vascular permeability and blood flow protective effects of sodium butyrate on rats following 50%TBSA full-thickness burns. Methods 48 SD rats, weighing 240-260 g, were randomly divided into three groups: sham group, scald group, scald + BTR group. Rats in scald group and scald + BTR group were subjected to 50% TBSA third-degree burns by immersing the back for 15 seconds and the abdomen for 8 seconds in boiling water. The sham group was immersed in 37 ℃ water instead. And then 1ml BTR (400 mg/kg) or 1ml normal saline was intraperitoneally injected, respectively. Blood flow in small intestine was measured by Doppler 3 and 6 hours after injury. Blood was drawn from the heart at 3 and 6 hours after injury for measurement of serum diamine oxidase (DAO); the small intestine tissues were harvested for the measurement of micro-vascular permeability and the changes in tissue water content. Results Compared with scald group, the activity of DAO [(43.76±9.34)U/L vs (73.29±11.34) U/L], the micro-vascular permeability [(4.26±0.98)μg/ml vs (5.53±1.31) μg/ml] and the rate of tissue water [(63.67±3.35)% vs (74.32±3.74)%] in scald + BTR group were significantly lower at 3 hours after scald injury (all P<0.05). In addition, BTR was shown to significantly increase the IMBF level [(67.21±9.47)BPU vs (55.18±10.48)BPU] induced by scald injury at 3 hours (P<0.05). The result at 6 hours after scald injury was similar to 3 hours. Conclusions Sodium butyrate has significant protective effects on small intestinal micro-vascular permeability and blood flow in rats with severe scald injury.

Key words： sodium butyrate scald small intestine tissue water content vascular permeability

Received: 20 August 2015

ZTFLH:

R644

Fund:1. 国家发改委卫星示范专项[发改办高技(2013)2140号]; 2. 国家自然科学基金(81471872)

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	Articles by authors
	TANG Fubo
	ZHENG Jinguang
	ZHANG Wenjing
	HU Sen
	and BAI Xiaodong

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TANG Fubo,ZHENG Jinguang,ZHANG Wenjing等. Effects of sodium butyrate on intestinal micro-vascular permeability and blood flow in rats with severe scald injury[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2016, 27(3): 246-249.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2016/V27/I3/246

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