早期大剂量地塞米松对挤压综合征大鼠模型心肌特异性损伤的疗效

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Abstract Objective To evaluate the effect of the early stage high-dose dexamethasone on cardiac-specific injury in the crush-syndrome rats.Methods CS rats were divided into three groups:CS group, NS group and DEX group (n=20 each). DEX group and NS group were intravenously administered 5mg/kg of dexamethasone and an equal volume of nomal saline respectively immediately before reperfusion while CS group received no treatment.A sham group serving as a blank control underwent the same procedures as all the three CS groups except for the compression and decompression and drug intervention. Results Myocardial specific injuries: (1) In serum cTnI OD, there were no statistically significant differences between all groups at 3 h after decompression. At 6h, the serum levels of cTnI increased in all three groups (compared with sham group), P<0.01. In NS group and DEX group were significantly lower than in CS group, and the P value was smaller than 0.05 and 0.01 respectively. There was no significant difference between DEX and NS groups. At 12 h in all three treatment groups were higher than in sham group (P<0.01).The differences between the three treatment groups were not statistically significant. At 24 h in group CS group and NS group were significantly higher than in sham group, P<0.01 and P<0.05 respectively.There was no significant difference between DEX group and sham group. In DEX group was significantly lower than in CS group, P<0.05. There were no significant differences between NS and DEX group, NS and CS group. (2) Apoptosis rate in myocardium: the apoptosis rate in CS group, NS group and DEX group at each time point were higher than that in sham group, P<0.01. At 3 h in NS group and DEX group were lower than in CS group, P<0.05 and P<0.01, respectively. There was no differences between NS and DEX group. At 6 h in NS group and DEX group were still lower than in CS group, P<0.05 and P<0.01, respectively. In DEX group were lower than in NS group, P<0.05.At 12 h and 24 h, there was no difference between NS and CS group. In DEX group were significantly lower than in NS group, P<0.01. (3) HE staining of myocardium: in sham group was normal. In the CS group, the congestive changes were observed and aggravated along with increasing time. NS group and DEX group had different degree of congestion and edema relief, and the relief in DEX group was more obvious.Conclusions There is cardiac specific injury in CS rat model, which may be threated by high-dose dexamethasone and normal saline. High-dose dexamethasone is more effective than an equal volume of normal saline. High dose of glucocorticoid may reduce the myocardial injury in crush syndrome rats through its suppression of systemic inflammatory response.

Key words： crush syndrome rats inflammation cardiac-specific injury dexamethasone

Received: 11 August 2015

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	Articles by authors
	YU Sijun
	YAN Zheng
	LU Jing
	JIN Dekui
	ZHANG Cong
	and LIU Huiliang

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YU Sijun,YAN Zheng,LU Jing等. Effect of early stage high-dose dexamethasone on cardiac-specific injury in crush-syndrome rats[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2016, 27(3): 281-284.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2016/V27/I3/281

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