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| Effect of intermittent hypoxia on transverse aortic constriction of intestinal flora in a murine model |
| ZHOU Xianjie1,LI Jun2, LU Feixiang1, XIA Jingyuan1, and LIU Qingchun1 |
1.General Hospital of Chinese People’s Armed Police Force,Beijing 100039,China;
2.Laboratory Animal Center, Peking University, Beijing 100871, China |
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Abstract Objective To study the influence of intermittent hypoxia on transverse aortic constriction (TAC) of intestinal flora in a murine model.Methods Eighteen C57BL/6 mice were evenly and randomly divided into three groups: Group C, Group TC and Group TH. Twelve of these mice were subjected to transverse aortic constriction and six model mice to a pattern of intermittent hypoxia (8 h at 10.5% O2 and 16 h at room air) for 10 days. Twelve mice served as normoxic controls. Faecal samples were obtained and microbiome composition was determined by 16 S rDNA pyrosequencing and bioinformatics analysis by Quantitative Insights into Microbial Ecology.Results A lower abundance of Syntrophobacteraceae was detected in the normoxic-exposed transverse aortic constriction model mice than in controls.The difference between TC and C was of statistical significance (P=0.004), but was P=0.013 between TC and TH.Conclusion sIntermittent hypoxia can alter intestinal flora in a mouse model. Whether this change has influence on the progress of the transverse aortic constriction needs to be further studied.
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Received: 11 May 2016
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2016, Vol. 27 
