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| Qualitative identification and content determination of ferulic acid in Yansheng capsules |
| LI Hua and WANG Xiaohong |
| Pharmacy Department, Inner Mongolia Autonomous Region Corps Hospital, Chinese People’s Armed Police Force, Huhhot 010040, China |
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Abstract Objective To determine the content of ferulic acid in Yansheng capsules in order to provide reference for pharmaceutical production. Methods TLC was used to identify radix angelicae sinensis and rhizoma ligustici wallichii while high performance liquid chromatography(HPLC)was used for determination of ferulic acid. Chromatographic conditions for ferulic acid included a mobile phase composed of methanol and 1% ice acetic acid solution(30∶70), room temperature, and a detection wavelength of 320 nm. Results The result of TLC showed that the identification spots were clear and negative without any interference observed under a 365 mm UV-lamp using a silican gel layer and with ethane and ice acetic acid (16∶16∶2) as the solvent. Ferulic acid showed a good linearity and precision within the rangeof 0.2515-1.509 μg/μl, r=0.9995. The RSD of the constrast solution and the supplied test-solution was 0.35% and 0.73%, respectively. The average recovery was 99.22%, with an RSD of 1.4%. The supplied test-solution was basically stable within 6 hours. Conclusions This method is simple, accurate and reproducible, which can be used for quality control of Yansheng capsules.
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Received: 20 June 2016
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| [1] |
王东风,黄 娜. 薄层扫描法测定舒瘀丹中阿魏酸的含量[J]. 中国民族民间医药,2012,21(18):58-59.
|
| [2] |
国家药典委员会. 中华人民共和国药典(2015年版第四部)[M].北京:中国医药科技出版社,2015:25.
|
| [3] |
国家药典委员会. 中华人民共和国药典(2015年版第一部)[M].北京:中国医药科技出版社,2015:245.
|
| [4] |
陈燕飞,刘乐乐,王玉华,等. 薄层色谱法鉴定冬葵子中阿魏酸[J].内蒙古医学院学报, 2008,30(2):115-117.
|
| [5] |
穆惠军,关桂菊,高 琴. 心脑通胶囊中阿魏酸的定性鉴别与含量测定[J]. 山东中医杂志,2002, 21(8):496-498.
|
| [6] |
秦 龙, 李 平,杨奇明,等.当归保肝胶囊的质量标准研究[J].兰州大学学报(医学版), 2016,42 (2): 5-10.
|
| [7] |
顾秀琰,王雪梅,王 昕.当归、川芎不同配比中阿魏酸的含量测定[J].卫生职业教育,2015,33(22):78-80.
|
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2016, Vol. 27 
