武威市某镇乙型肝炎感染者家庭内部隐匿性感染现状及其分子进化特征

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Abstract Objective To study the status quo and molecular evolution of occult infection in intrafamilial patients with hepatitis B virus infection in the city of Wuwei.Methods All the people who were found to be infected with HBsAg during a physical examination in 2009 in a town in Wuwei were questioned for their HBV family history. After they signed informed consent, their blood was drawn and tested for hepatitis B surface antigen (HBsAg), antibody to HBsAg (anti-HBs), hepatitis B e-antigen (HBeAg), antibody to HBe (anti-HBe) and antibody to hepatitis B core antigen (anti-HBc) using commercially available enzyme-linked immunosorbent assay (ELISA). DNA was extracted using magnetic Beads extraction kit from all the samples, and amplification was performed by nested PCR. Sequences obtained were analyzed by mega 6.0.Results Of the 80 serum samples, the positive rate of HBsAg, Anti-HBc, and HBV DNA was 43.8%, 78.8% and 37.5%, respectively. The rate of infection with intrafamilial occult hepatitis B was 26.7%(12/45).Conclusions The prevalence of intrafamilial occult hepatitis B infection is significantly higher than in the general population. The main genotype is genotype C and genotype D in particular.

Key words： hepatitis b virus occult virus infection molecular evolution

Received: 20 July 2016

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R512.62

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	Articles by authors
	WEN Xiaohui
	PU Zhongshu
	WANGY Yang
	FU Ting
	CHENG Huijuan
	SU Haixia
	and SHAO Zhongjun

Cite this article:

WEN Xiaohui,PU Zhongshu,WANGY Yang等. The status quo and molecular evolution of occult infection in intrafamilial patients with hepatitis B virus infection in Wuwei[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2017, 28(2): 146-149.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2017/V28/I2/146

[1]	Van Damme P, Cramm M, Van der Auwera J C, et al. Horizontal transmission of hepatitis B virus [J]. Lancet, 1995, 345(8941): 27-29.
[2]	Raimondo G, Allain J, Brunetto M R, et al. Statements from the Taormina expert meeting on occult hepatitis B virus infection [J]. J Hepatol, 2008, 49(4): 652-657.
[3]	Kwak M. Occult hepatitis B virus infection [J]. World J Hepatol, 2014, 6(12): 860.
[4]	Chan H L, Tsang S W, Leung N W, et al. Occult HBV infection in cryptogenic liver cirrhosis in an area with high prevalence of HBV infection [J]. Am J Gastroenterol, 2002, 97(5): 1211-1215.
[5]	Simonetti J, Bulkow L, McMahon B J, et al. Clearance of hepatitis B surface antigen and risk of hepatocellular carcinoma in a cohort chronically infected with hepatitis B virus [J]. Hepatology, 2010, 51(5): 1531-1537.
[6]	Huang X, Hollinger F B. Occult hepatitis B virus infection and hepatocellular carcinoma: a systematic review [J]. J Viral Hepat, 2014, 21(3):153-162.
[7]	Kitab B, Ezzikouri S, Alaoui R, et al. Occult HBV infection in Morocco: from chronic hepatitis to hepatocellular carcinoma [J]. Liver Int, 2014,34(6):e144-e150.
[8]	何英利,赵英仁,高珩,等. 聚集性感染家族内乙型肝炎病毒标志物[J]. 第四军医大学学报, 2006, 27(24): 2288-2290.
[9]	Zhang Z, Zhang L, Dai Y, et al. Occult hepatitis B virus infection among people with a family history of chronic hepatitis B virus infection [J]. J Med Virol, 2015, 87(11): 1890-1898.
[10]	颜丙玉,张丽,纪峰,等. 山东省社区人群隐匿性乙型肝炎病毒感染现状分析[J]. 中国疫苗和免疫, 2011, 17(4): 303-306.
[11]	Fang Y, Shang Q, Liu J, et al. Prevalence of occult hepatitis B virus infection among hepatopathy patients and healthy people in China [J]. J Infect, 2009, 58(5): 383-388.
[12]	吉兆华,张琪,闫永平,等. 甘肃省武威市人群乙肝流行现状及其影响因素研究[C]//中华预防医学会第四届学术年会论文集. 北京:人民卫生出版社,2013: 37-38.
[13]	Tong S, Revill P. Overview of hepatitis B viral replication and genetic variability [J]. J Hepatol, 2016, 64(1): S4-S16.
[14]	Zhu C T, Dong C L. Characteristics of general distribution of hepatitis B virus genotypes in China [J]. Hepatobiliary Pancreat Dis Int, 2009, 8(4): 397-401.
[15]	Rastegarvand N, Makvandi M, Samarbafzadeh A, et al. Molecular characterization of pre-core/core and sregion of hepatitis Bvirus in hemodialysis patients withoccult hepatitis Binfection [J]. Jundishapur J Microbiol, 2015, 8(10):987-995.
[16]	Nainan O V, Khristova M L, Byun K, et al. Genetic variation of hepatitis B surface antigen coding region among infants with chronic hepatitis B virus infection [J]. J Med Virol, 2002, 68(3): 319-327.