胎肾细胞悬液对去势大鼠骨组织不同部位ER-α表达的影响

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Abstract Objective To investigate the effect of FRCS on expression of bone ER-αin rats with ovariectomy so as to provide evidence for the theory that the kidney dominates bones.Methods Four-month-old Sprague Dawley female rats were randomly divided into sham operated group, model control group, estrogen treatment group and FRCS treatment group. Only the fat around the ovary was removed in sham operated group, while ovaries were removed in other groups. Four weeks after operation, estrogen and FRCS were administered to rats of treatment groups respectively, and the treatment lasted four weeks. Then, the bone morphology was evaluated by HE stain, the serum level of E₂ was detected by radio-immunity, the protein expression of ER-α in cells of the bone tissue surface was investigated by immunohistochemical method.Results Compared with sham operated group, the bone trabecula in model control group was sparce and disorderly, the trabecular interspace was enlarged more obviously, and the serum level of E₂ decreased(P<0.01). Compared with model control group, the serum level of E₂ in estrogen treatment group and FRCS treatment group increased(P<0.01)and the bone brabecula was more continual and aligned in better order, the uterus index changed as serum E₂ did. Compared with sham operated group, the expression of ER-α decreased in the articular cartilage, epiphyseal plate, bone trabecula and bone marrow(All P<0.05)in model control group. Compared with model control group, the expression of ER-α increased in estrogen treatment group and FRCS treatment group(All P<0.05).Conclusions The mechanism by which FRCS can prevent and cure postmenopausal osteoporosis may lie in the ability of FRCS to modulate the expression of bone ER-α.

Key words： fetal renal cell suspension ER-α postmenopausal osteoporosis

Received: 20 June 2016

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	Articles by authors
	GU Yong
	GUO Hui
	DA Chenxing
	and ZHENG Qinglian

Cite this article:

GU Yong,GUO Hui,DA Chenxing等. Effect of fetal renal cell suspension on expression of bone ER-α in rats with ovariectomy[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2017, 28(2): 157-160.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2017/V28/I2/157

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