免疫球蛋白含量的检测与分离技术探讨

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (437 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective To explore the detection methods of contents of immunoglobulin and separation technology.Methods Ninety cases of abnormal serum immunoglobulin treated in our hospital between October 2013 and October 2016 were collected and their serum samples were equally divided into three groups. The contents of IgG, IgA and IgM were detected by the immunodiffusion method, immune nephelometry method, and immune turbidimetric method respectively, while the abnormalities of IgG, IgA, IgM, and the content of immuneglobulin determined with the three detection methods were statistically analyzed.Results The abnormal rates of IgG, IgA and IgM by immunodiffusion method were 22.6%, 12.4% and 7.9% respectively, those by immune nephelometry method were 20.3%, 14% and 9.2% respectively, and those by immune turbidimetric method were 8%, 1.5% and 6.2% respectively.The abnormal rates of IgG, IgA and IgM detected by the single diffusion method and immune turbidimetric method were significantly higher than those by the immune turbidimetric method (P<0.05), but the difference of the abnormal rates of IgG, IgA and IgM between the single expansion method and immunoturbidimetry determination was not significant. The IgA, IgM contents detected by the single expansion method and immune turbidimetric method were significantly higher than those by the immune turbidimetric method (P<0.05), and the IgA and IgM contents detected by the immune nephelometry method were significantly higher than those by the single expansion method (P<0.05), but the difference of IgG contents between the three detection methods was not significant.Conclusions The effect of single expansion method and immune transmission turbidity method is better than that of immune transmission turbidity method.

Key words： immunoglobulin content detection and separation technology

Received: 20 September 2016

ZTFLH:

R53.3

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors

Cite this article:

URL:

http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2017/V28/I4/355

[1]	张晓晖.60例多发性骨髓瘤免疫固定电泳及免疫球蛋白分析[J].现代中西医结合杂志,2012,21(6):608-609.
[2]	张慧,周莉莉,李荣,等.免疫球蛋白重／轻链检测在IgG型多发性骨髓瘤患者微小残留病监测中的作用[J].中华血液学杂志,2015,36(2):95-98.
[3]	张小杰,周健.免疫固定电泳技术在多发性骨髓瘤诊断中的应用[J].中国实用医刊,2013,40(14):71-73.
[4]	柏丹丹,李红卫,潘莉莉,等.荧光高效液相色谱法测定血清中总唾液酸含量的分析[J]. 中国儿童保健杂志,2012,20(11)1022-1025.
[5]	阳洋,苏梦宁,杨如意.母婴血清和母乳中免疫球蛋白含量的检测分析与检验学研究[J].中华医学检验杂志,2015,38(1):68-69.
[6]	满江红,吴俊贵,苏畅.母亲和婴儿血清与母乳中免疫球蛋白含量分析与临床检验学研究[J].中华医学检验杂志,2015,38(2):91-93.
[7]	江山,苏宁,阳洋.母婴血清和母乳中免疫球蛋白含量的检测与临床意义[J].国外医学,临床生物化学与检验学分册,2015,36(3):178-182.
[8]	熊政,崔玉波,田玉旺.提高细胞化学染色的方法与应用[J].医学研究通讯,2014,42(1):33-36.
[9]	王燕,高惠红,王海鹰,等.临床细菌检验与预防医院感染[J].标记免疫分析与临床,2015,22(2):21-22.
[10]	熊政.免疫组织化学中抗原修复的研究进展[J].中华病理学杂志,2015,44(1):42-44.
[11]	李金明,郑怀竞.临床ELISA试剂盒的选用和质检[J].临床检验信息导报,2015,22(2):57-58.
[12]	李健斋.血液载脂蛋白免疫测定及其标准化问题[J].中华医学检验杂志,2014,37(4):253-255.
[13]	Bendo L,Casanova M,Figueira A C, et al.Nanostructured sensors containing immobilized nuclear receptors for thyroid hormone detection[J]. J Biomed Nanotechnol, 2014, 10 (5):744-750.
[14]	Heinrich J. Huber, Paul Holvoet.Exosomes: emerging roles in communication between blood cells and vascular tissues during atherosclerosis[J].Current Opinion in Lipidology, 2015, 26 (5):412-419.
[15]	Maria Giovanna Danieli,Federica Pulvirenti, Valeria Rocchi,et al.Self-administered hyaluronidase-facilitated subcutaneous immunoglobulin therapy in complicated primary antibody deficiencies[J].Immunotherapy, 2016, 8 (9):995-1002.
[16]	Carne E,Ponsford M,ElShanawany,et al.Five years of self-administered hyaluronidase facilitated subcutaneous immunoglobulin (fSCIg) home therapy in a patient with primary immunodeficiency[J].J Clin Pathol, 2016, 69 (1):87-88.
[17]	Raja J,Nihtyanova S I,Murray C D,et al.Sustained benefit from intravenous immunoglobulin therapy for gastrointestinal involvement in systemic sclerosis[J].Rheum, 2016, 55 (1):115-119.