嗅鞘细胞移植与α晶体蛋白联合对大鼠视神经损伤后视功能恢复的促进作用

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Abstract Objective To investigate the synergistic effect of olfactory ensheating cells (OECs) and α-crystallin on visual function recovery of nerve injury in adult rats.Methods α-crystallin was injected into the vitreous cavity, and OECs were transplanted to the area of optic nerve injury. The function of optic nerves was assessed by flash-elicited visual-evoked potential(FVEP) at 1 and 2 weeks, 1 and 3 months, and immediately after OECs transplantation and α-crystallin injection.Results Compared to PBS, OECs, the combination of OECs and α-crystallin promoted the recovery of the amplitude of P1 wave at 1 week 【(8.49±1.19)μv, (9.33±2.54)μv】, 2 weeks 【(8.85±1.92)μv,(9.43±2.41)μv】,1 month 【(8.46±1.09)μv,(8.72±1.91)μv】, 2 months 【(8.12±1.41)μv,(8.48±1.67)μv】 and 3 months 【(7.68±2.56)μv,(8.08±1.47)μv】 after optic nerve injury (P<0.01). The recovery of the amplitude of P1 wave was much more significantin the combination of OECs and α-crystallin group than in other groups. In α-crystallin group, the amplitude of P1 wave partly recovered after 1 and 2 weeks, but decreased after 1 month, and was not significantly different from that of the PBS group after 3 months. The latency of P1 wave did not recover in OECs group, α-crystallin group, or in the combination group.Conclusions Compared to OECs and α-crystallin alone, the effect of the combination of OECs and α-crystallin on functional recovery is significant.

Key words： α-crystallin olfactory ensheathing cells visual functional recovery

Received: 20 January 2017

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