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| Identification and functional analysis of Rab25-interacting proteins in gastric cancer cells |
| WANG Yicheng1 and LI Yuanyuan2 |
1. Department of Servicemen,Chongqing Municipal Corps Hospital,Chinese People’s Armed Police Force, Chongqing 400061,China;
2.Air Force Aviation Medicine Identification and Training Center, Dalian 116013, China |
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Abstract Objective To identify Rab25-interacting proteins in gastric cancer cells using tandem affinity purification-mass spectrometry (TAP-MS).Methods An SGC7901 cell strain that could stably express Rab25 was constructed using a StrepII-6His-EGFP-Rab25 expressing plasmid by lentivirus transfection technology. After StrepII-6His tandem affinity purification, mass spectrometry was carried out to analyze the interaction protein complex of Rab25. To further verify their interactions, a protein-protein network was constructed and the authenticity of interactions was verified by co-immunoprecipitation in gastric cancer cells.Results A total of 26 Rab25 interacting proteins were identified by TAP-MS technique, which were enriched in the cancer process, apoptosis, NOTCH-mediated pathway and microtubule-based movement. Co-immunoprecipitation analysis confirmed that Itgb1 interacted with Rab25, and that they collaborated to promote the invasion of SGC7901 cells according to Transwell invasion assays.Conclusions This study helps to draw a detailed map of Rab25 interactions and provides clues for treatment of gastric cancer.
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Received: 02 March 2017
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| [1] |
Nagini S. Carcinoma of the stomach: a review of epidemiology, pathogenesis, molecular genetics and chemoprevention [J]. World J Gastrointest Oncol, 2012, 4(7): 156-169.
|
| [2] |
Bhuin T, Roy J K. Rab proteins: the key regulators of intracellular vesicle transport [J]. Exp Cell Res, 2014, 328(1): 1-19.
|
| [3] |
Wang J, Cui S, Zhang X, et al. High expression of heat shock protein 90 is associated with tumor aggressiveness and poor prognosis in patients with advanced gastric cancer [J]. PLoS One, 2013, 8(4): e62876-e62876.
|
| [4] |
He L J, Xie D, Hu P J, et al. Macrophage migration inhibitory factor as a potential prognostic factor in gastric cancer [J]. World J Gastroentero, 2015, 21(34): 9916-9926.
|
| [5] |
LeRoy G, Rickards B, Flint S. The double bromodomain proteins Brd2 and Brd3 couple histone acetylation to transcription [J]. Mol Cell, 2008, 30(1): 51-60.
|
| [6] |
Kimura M. IRF2-binding protein-1 is a JDP2 ubiquitin ligase and an inhibitor of ATF2-dependent transcription [J]. FEBS Lett, 2008, 582(19): 2833-2837.
|
| [7] |
Howe J, Sayed M, Ahmed A, et al. The prevalence of MADH4 and BMPR1A mutations in juvenile polyposis and absence of BMPR2, BMPR1B, and ACVR1 mutations [J]. J Med Genet, 2004, 41(7): 484-491.
|
| [8] |
Salton M, Lerenthal Y, Wang S Y, et al. Involvement of Matrin 3 and SFPQ/NONO in the DNA damage response [J]. Cell Cycle, 2010, 9(8): 1568-1576.
|
| [9] |
Desgrosellier J S, Cheresh D A. Integrins in cancer: biological implications and therapeutic opportunities [J]. Nat Rev Cancer, 2010, 10(1): 9-22.
|
| [10] |
Dozynkiewicz M A, Jamieson N B, MacPherson I, et al. Rab25and CLIC3 collaborate to promote integrin recycling from late endosomes/lysosomes and drive cancer progression [J]. Dev Cell,2012,22(1):131-145.
|
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2017, Vol. 28 
