手足口病病原体EV71的检测方法学比较

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Abstract Objective To compare the effects of four different methods in the detection of pathogen enterovirus type 71 (EV71)of hand-foot and mouth disease (HFMD), and to provide reference for clinical detection.Methods African green monkey cells (Vero) were cultured until the logarithmic growth phase before they were infected with the EV71 strain. The cytopathic effect (CPE) method, real-time polymerase chain reaction (Real-Time PCR), Western blot and immunofluorescence were used respectively to detect the infection of EV71.The results of the four tests were compared.Results All the four methods were able to detect the infection of EV71. Among them, CPE was the most economical method, but its detection cycle was so long as to take at least 96 hours, and it required good vitality of the virus. The effect of detection of Western blot and immunofluorescence was similar, and the experiment could be completed in 72 hours, but their sensitivity was low and a relatively large amount of virus was required. Real-Time PCR detection was both quick and highly sensitive, and the experiment could be completed in 48 hours.Conclusions Each method has its own advantages and disadvantages.A method should be chosen according to different needs so as to make quick and accurate diagnosis of pathogens.

Key words： hand-foot and mouth disease EV71 cytopathic effect Real-Time PCR immunofluorescence

Received: 22 August 2017

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	FU Hui
	AN Lina
	PENG Bibo

Cite this article:

FU Hui,AN Lina,PENG Bibo. Comparison of detection methods of pathogen EV71 in hand-foot and mouth disease[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2018, 29(1): 60-63.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2018/V29/I1/60

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