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| Biological imaging effect and biocompatibility of graphene quantum dots (GQDs) of different size in PC12 cells |
| DENG Banglian1,2, CHEN Xuefeng1, MENG Lei1, YI Zian1, QIN Wen3, LIU Yan3, SONG Shuang4, PENG Chao1, and JU Yanjing1. |
1.Department of Orthodontics, Lianbang Dental Hospital of Xincheng District, Xi’an 710000,China;
2.Department of Stomatology, People’s Hospital of Ankang, Ankang 725000,China;
3.Department of Orthodontics, School of Stomatology, the Fourth Military Medical University, Xi’an 710032 China;
4.Peking University School of Stomatology, Beijing 100191,China |
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Abstract Objective To evaluate the biological imaging effect and biocompatibility of graphene quantum dots (GQDs) of different size in PC12 cells.Methods The hydrodynamic size and zeta potencial of GQDs in distilled water (DW) were determined by dynamic light scattering (DLS). Furthermore, cell survival rates following treatment with GQDs were evaluated using the CCK-8 assay and the biological imaging effect of GQDs in PC12 cells was observed under a confocal microscope.Results The toxic effect of GQDs in PC12 cells was size-dependent. The results of CCK-8 assay indicated that 15 nm GQDs showed lower toxicity than CDs in PC12 cells. After PC12 cells were incubated 48 h with 500 μg/ml GQDs, cell viability remained at 80% or more. What’s more, 15 nm GQDs could be taken up by PC12 cells more easily and more than 80% cells could be labeled successfully, which indicated good biological imaging effects.Conclusions GQDs have low cytotoxicity and excellent biological imaging properties, which are a suitable nanomaterial for neuroimaging.
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Received: 15 April 2018
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2018, Vol. 29 
