小RNA干扰沉默S100A13对甲状腺细胞侵袭、迁移的影响及机制

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Abstract Objective To investigate the effect of interference with the expression of calcium binding protein A13 (S100A13) on the invasion and migration of thyroid carcinoma TPC-1 cells and the possible mechanism.Methods The expression of S100A13 gene in thyroid cancer TPC-1 cells was knocked down by siRNA. The effect of S100A13 on the migration of TPC-1 cells was evaluated with the wound healing scratch test and Transwell assay. The effect of S100A13 on the invasiveness of TPC-1 cells was assessed by Transwell invasion test. The expressions of S100A13, MMP-2, E-cadherin and vimentin were detected by Western Blot.Results Compared with the control group, the silencing of S100A13 by siRNA in human thyroid carcinoma TPC-1 cells significantly increased the scratch width(25.214±2.687 vs 69.223±5.291), but the ability of invasion(0.897±0.082 vs 0.521±0.053) and migration(1.263±0.252 vs 0.759±0.062)decreased significantly (P<0.05). The levels of MMP-2 (0.523±0.062 vs 0.246±0.033)and vimentin(0.863±0.082 vs 0.458±0.053) in S100A13-treated cells were significantly decreased (P<0.05), while E-cadherin protein(0.445±0.053 vs 0.755±0.078)was significantly increased (P<0.05).Conclusions Interference with S100A13 expressions by siRNA inhibits the invasion and migration of thyroid carcinoma TPC-1 cells, which may be related to the inhibition of EMT and MMP-2.

Key words： thyroid cancer S100A13 invasion migration

Received: 01 June 2018

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	Articles by authors
	LI Jian
	YAN Shuangtong
	GONG Yanping
	LU Yanhui
	SUN Banruo
	CHENG Xiaoling
	SHAO Yinghong

Cite this article:

LI Jian,YAN Shuangtong,GONG Yanping等. Effect and mechanism of sliencing S100A4 by small RNA interference of S100A13 on thyroid cells[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2018, 29(10): 960-963.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2018/V29/I10/960

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