|
|
|
| Determination of resveratrol and ε-viniferincontents in the stems of grape vines from different habitats |
| SONG Fei1, HE Lingshuang2, SUN Huageng2, ZHAO Yanmin2,3, LIU Dan4, FU Shan2, LIU Dailin3 |
1.Meidical and Research Department,Characteristic Medical Center of PAP, Tianjin 300162 , China;
2.School of Chinese Meteria Medic,Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;
3.Pharmaceutical Department,Logistics University of PAP ,Tianjin 300309 , China;
4.Tianjin Jian Feng Natural Product R&D Co.,Ltd, Tianjin 300457, China |
|
|
|
|
Abstract Objective To determine the contents of resveratrol and ε-viniferin in the stems of muscat grape vines grown in different areas.Methods High performance liquid chromatography was applied to determine the contents with a mobile phase that consisted of methanol-acetic acid aqueous solution by gradient elution. The wavelength of detection was 310 nm and 254 nm respectively, the flow rate was 1 mL/min, and the column temperature was 30 ℃. Results The linear relationships of resveratrol and ε-viniferin were good in the range of 1.76 to 17.6 μg and 2.75 to 27.54 μg respectively(R2=0.9999). The contents of resveratrol and ε-viniferin determined with this method ranged from 1.94 to 5.96μg/g and 8.74 to 15.41 μg/g in ten samples, while their coefficients of variation were 40.64% and 17.67%, respectively.Conclusions This method is simple, accurate and can be used to for quality control of grape vines.
|
|
Received: 19 November 2018
|
|
|
|
|
|
| [1] |
李先宽,李赫宇,李 帅,等. 白藜芦醇研究进展[J]. 中草药,2016,47(14):2568-2578.
|
| [2] |
谢红旗,刘发宝,杨星星,等.葡萄植株中白藜芦醇及葡萄素含量动态积累规律研究[J].天然产物研究与开发,2012,24(8):1079-1083.
|
| [3] |
王琴飞,马丽艳,黄绵佳,等.高效液相色谱法测定葡萄叶中5种茋化物[J]. 分析化学研究简报,2008,36(10):1359-1363.
|
| [4] |
Yao Ch S, Huang K S, Lin M, et al .A new stilbene dimer from vitisamurensis[J]. J Asian Nat Prod Res, 2013, 15(6): 693-695.
|
| [5] |
Chou W F, Shen C C.Oligostilbenes from the roots of vitisthunbergii[J]. Planta Med. 2009, 75: 856-859.
|
| [6] |
Huang K S, Lin M, Yu L N,et al.Four novel oligostilbenes from the roots of vitisamurensis[J]. Tetrahedron, 2000, 56:1321-1329.
|
| [7] |
Nahla Z,Shigeki Y,Masahiro A, et al. Greater effectiveness of ε-viniferin in red wine than its monomer resveratrol for inhibiting vascular smooth muscle cell proliferation and migration[J]. Biosci Biotechnol Biochem, 2011,75(7): 1259-1267.
|
| [8] |
周晓明,郭春妙,樊丁宇,等. HPLC法测定不同品种葡萄及葡萄干白藜芦醇含量[J].新疆农业科学,2013,50(12):2223-2229.
|
| [9] |
陈梦微,邓群仙,张金容,等. 葡萄不同品种和组织中白藜芦醇含量及其抗氧化活性分析[J].食品与机械,2017,33(1):150-154.
|
| [10] |
陈 雷,韩雅珊.葡萄不同品种和组织白藜芦醇含量的差异[J].园艺学报,1999,26(2):50-51.
|
| [11] |
吴艳丽,杨鸿雁,郭君钦.HPLC法测定山葡萄藤中白藜芦醇的含量[J].临床医药文献杂志,2017, 38(4):7499.
|
| [12] |
杨荣艳,刘琬晖,高楠楠.HPLC法测定山葡萄藤中白藜芦醇含量[J].中国卫生产业,2018,15:6-8.
|
| [13] |
谢红旗,杨星星,曾建国. 不同前处理方法对鲜葡萄枝蔓中茋类化合物的影响研究[J].中草药,2014,45(24): 3553-3558.
|
| [14] |
Liu M H,Lin X L,Li J,et al.Resveratrol induces apoptosis through modulation of the Akt / FoxO3a / Bim pathway in HepG2 cells[J].Mol Med Rep,2016,13: 1689-1694.
|
| [15] |
Nikhil K,Sharan S,Singh AK,et al.Anticancer activities of pterostilbene-isothiocyanate conjugate in breast cancer cells: involvement of PPARγ. [J].PLoS One,2014,9:e104592.
|
| [16] |
冯 苗. 白藜芦醇抑制人结肠癌细胞增殖和诱导调亡及与cox-2关系的研究[D].南昌:南昌大学第一附属医院,2016.
|
| [17] |
安 梅,周 瑾,陈晓宇. 白藜芦醇药理学作用的研究进展[J].肿瘤药学,2014,4(4):242-246.
|
| [18] |
邓金华,张奉莲,王定勇,等.肾康注射液治疗慢性肾衰竭的Meta分析[J].现代药物与临床,2015,30(5):595-599.
|
| [19] |
张 倩,柏 帅,李克文,等.白藜芦醇研究进展及现状[J].精细雨专用化学品,2017,25(10):39-41.
|
| [20] |
孙治刚,李志娜,李 敏.白藜芦醇的药理作用研究进展[J]. 淮海工学院学报(自然科学版),2017,26(2):40-43.
|
|
|
|
2019, Vol. 30 
