过表达miR-26b-5p抑制HMGA1参与卵巢癌细胞上皮间质转化的机制

Abstract
Figure/Table
References
Related Citation (2)

Download: PDF (685 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective To explore the mechanism by which microRNA-26b-5p (miR-26b-5p) mediates high-mobility group A1 (HMGA1) in epithelial-mesenchymal transformation of ovarian cancer.Methods The target gene of microRNA-26b-5p was predicted. The expressions of miR-26b-5p and HMGA1 in tissues were detected by real-time reverse transcription PCR (qRT-PCR). After grouping and transfection of ovarian cancer cells, the expressions of mRNA and protein of relevant factors were detected by qRT-PCR and Western blot in the cells. Transwell invasion test was used to detect cell invasion and scratch test to detect migration ability, while qRT-PCR and Western blot were used to detect the expressions of E-cadherin and Vimentin genes related to epithelial-mesenchymal transformation in tumors.Results The expression of miR-26b-5p in ovarian cancer tissue decreased, while the expression of HMGA1 increased (All P＜0.05). miR-26b-5p mimic and sh-HMGA1 groups showed decreased expressions of HMGA1 and Vimentin, increased expression of E-cadherin, and reduced cell invasion and migration (All P＜0.05). Co-transfection of miR-26b-5p inhibitor and sh-HMGA1 reversed the inhibitory effect of sh-HMGA1 on epithelial-mesenchymal transformation (All P＜0.05).Conclusions Over-expression of miR-26b-5p inhibits the expressions of HMGA1 and Vimentin in ovarian cancer cells, promotes E-cadherin expression, reduces cell invasion and migration, thus inhibiting epithelial-mesenchymal transformation of ovarian cancer cells.

Key words： ovarian cancer cell miR-26b-5p HMGA1 Vimentin E-cadherin epithelial-mesenchymal transformation

Received: 25 March 2019

ZTFLH:

R737.31

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	CAO Wenhong

Cite this article:

CAO Wenhong. Over-expression of MiR-26b-5p inhibits HMGA1 expression and participates in epithelial-mesenchymal transformation of ovarian cancer cells[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2019, 30(9): 760-763.

URL:

http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2019/V30/I9/760

[1]	Reid B M, Permuth J B, Sellers T A. Epidemiology of ovarian cancer: a review[J]. Cancer Bio Med, 2017, 14(1):9-32.
[2]	Huo W, Zhao G, Yin J, et al. Lentiviral CRISPR/Cas9 vector mediated miR-21 gene editing inhibits the epithelial to mesenchymal transition in ovarian cancer cells[J]. J Cancer, 2017, 8(1):57.
[3]	崔洪亮, 张阳德, 任菲,等. dbDEMC2.0:人类癌症相关miRNA数据库2.0[J]. 中国现代医学杂志, 2014, 24(3):77-79.
[4]	Piotrowskidaspit A S, Tien J, Nelson C M. Interstitial fluid pressure regulates collective invasion in engineered human breast tumors via Snail, vimentin, and E-cadherin[J]. Integr Biol, 2016, 8(3):319-331.
[5]	Yamashita N, Tokunaga E, Inoue Y, et al. Epithelial paradox; clinical significance of co-expression of E-cadherin and vimentin in the invasion and the metastasis of breast cancer[J]. Cancer Res, 2017, 17(4):6-17.
[6]	Lin J, Zhang L, Huang H, et al. MiR-26b/KPNA2 axis inhibits epithelial ovarian carcinoma proliferation and metastasis through downregulating OCT4[J]. Oncotarget, 2015, 6(27):23793-23806.
[7]	李励, 冯浩, 刘胜楠,等. miR-26b在卵巢癌组织中的表达水平及其与预后的关系[J]. 临床和实验医学杂志, 2017, 16(17):1700-1703.
[8]	张英芝. HMGA1在卵巢上皮性癌组织中的表达及临床意义[J]. 肿瘤学杂志, 2014, 20(1):51-54.
[9]	Wu T, Chen W, Liu S, et al. Huaier suppresses proliferation and induces apoptosis in human pulmonary cancer cells via upregulation of miR-26b-5p[J]. Febs Lett, 2014, 588(12):2107-2114.
[10]	Liu C Y, Lin H H, Tang M J, et al. Vimentin contributes to epithelial-mesenchymal transition cancer cell mechanics by mediating cytoskeletal organization and focal adhesion maturation[J]. Oncotarget, 2015, 6(18):15966-15983.
[11]	Zhu G, Zhu G, Song P, et al. Role of epithelial-mesenchymal transition markers E-cadherin, N-cadherin, β-catenin and ZEB2 in laryngeal squamous cell carcinoma[J]. Oncol Lett, 2018, 15(3):3472-3481.
[12]	柳培雨, 田毅, 鲍缦夕,等. 大鼠心肌缺血-再灌注损伤miRNA-214介导电针预处理的保护作用[J]. 武警医学, 2016, 27(1):43-47.
[13]	Li W, Zhang X, Ji W, et al. TGFβ1 in fibroblasts-derived exosomes promotes epithelial-mesenchymal transition of ovarian cancer cells[J]. Oncotarget, 2017, 8(56):96035-96047.
[14]	Park G B, Ko H, Kim D. Sorafenib controls the epithelial-mesenchymal transition of ovarian cancer cells via EGF and the CD44-HA signaling pathway in a cell type-dependent manner[J]. Mol Med Rep, 2017, 16(2):1826-1836.