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| Simultaneous determination of ferulic acid and glycyrrhizic acid in Xiao Yao Wan by HPLC |
| TIAN Jie1, SUN Yukun2, YIN Lu2, TANG Zhe2, and WANG Haipeng2 |
1.Department of Pharmacy,Chinese Medicine Hospital of Tangshan,Tangshan 063000, China;
2. Department of Pharmacy,Liaoning Provincial Crops Hospital,Chinese People’s Armed Police Force,Shenyang 110034,China |
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Abstract Objective To establish a method for the determination of ferulic acid and glycyrrhizic acid in Xiao Yao Wan.Methods The separation was performed on an Agilent Zorbax XDB-C18 column(150 mm×4.6 mm,5 μm)with acetonitrile/ phosphoric acid solution(0.05%) as the mobile phase with gradient elution at the flow rate of 1.0 ml/min. The detection wavelength was set at 278 nm, and the column temperature was 35 ℃.Results The linear range of ferulic acid and glycyrrhizic acid was 0.054-0.596 μg (r=0.9995) and 0.110-1.217 μg (r=0.9993) respectively. The average recovery was 99.29％(RSD=1.28％, n=6) and 98.34％(RSD=1.61％,n=6) respectively.Conclusions The established HPLC method has proved to be user-friendly, reproducible, precise and suitable for the content determination of Xiao Yao Wan.
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Received: 08 May 2019
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2019, Vol. 30 
