|
|
|
| Effects of methane-rich saline on liver mitochondria |
| ZHU Kaimin1, SHEN Meihua2, WANG Chengwei2, GAO Jing3, CHEN Yan2, and YANG Tao1 |
1.Department of Anesthesia,the Second Military Medical University,Shanghai 200433,China;
2.Intensive Care Unit,Shanghai Municipal Corps Hospital,Chinese People’s Armed Police Force,Shanghai 201103,China;
3.Institute of Mitochondrial Biology and Medicine, School of Life Science and Technology,Xi’an Jiaotong University,Xi’an 710049,China |
|
|
|
|
Abstract Objective To study the effect of methane on liver mitochondria in a mouse model of liver ischemia reperfusion.Methods C57 mice were randomly divided into the blank control group (SHAM group n=6), ischemia-reperfusion group ( IR group n=6 ) and methane-rich saline treatment group (IR+CH4 group n=6). The 70% liver ischemia-reperfusion model was adopted. Methane-rich saline 10ml/kg was injected intraperitoneally before reperfusion in the IR+CH4 group. Samples were taken to detect oxidation, antioxidation and mitochondrium related indexes.Results Methane-rich saline increased SOD(t=3.480, P=0.0037)/(GSHt=2.687, P=0.0177) levels but decreased MDA(t=2.771, P=0.015)/ROS(t=3.154, P=0.0083) levels. In the IR group, the expressions of mitochondrial fusion proteins Mfn1(q=7.57,P<0.05) and OPA1(q=6.41,P<0.05) decreased while the expression of mitogen DLP1(q=3.718,P<0.05) increased. PINK1 expression was up-regulated in the IR group (q=4.606,P<0.05)and down-regulated in the IR+CH4 group(q=3.922,P<0.05).Conclusions Methane-rich saline can improve the body’s antioxidant capacity, promote mitochondrial fusion, reduce mitochondrion, and boost the recovery of mitochondrial function.
|
|
Received: 08 March 2019
|
|
|
|
|
|
| [1] |
Keppler F, Hamilton J T, Braβ M, et al. Methane emissions from terrestrial plants under aerobic conditions [J] . Nature, 2006, 439(7073):187-191.
|
| [2] |
Wang Z P, Gulledge J, Zheng J Q, et al. Physical injury stimulates aerobic methane emissions from terrestrial plants[J]. Biogeosciences, 2009, 6:615-621.
|
| [3] |
Keppler F, Hamilton J T, McRoberts W C, et al.Methoxyl groups of plant pectin as a percursor of atmospheric methane: evidencedromdeuterium l abelling studies[J]. New Phytol, 2008, 178: 808-814.
|
| [4] |
Ghyczy M, Torday C, Kaszaki J, et al. Hypoxia-Induced generation of methane in mitochondria and eukaryotic cells-an alternative approach to methanogenesis[J]. Cell Physiol Biochem, 2008,21(3):251-258.
|
| [5] |
Tuboly E, Szabo A, Garab D, et al. Methane biogenesis during sodium azide-induced chemical hypoxia in rats[J]. Am J Physiol Cell Physiol, 2013,304(2): C207-214.
|
| [6] |
Wishkerman A, Greiner S, Ghyczy M, et al. Enhanced formation of methane in plant cell cultures by inhibition of cytochrome c oxidase[J]. Plant Cell Environ, 2011, 34(3): 457-64.
|
| [7] |
Tuboly E, Molnar R, Tokes T, et al. Excessive alcohol consumption induces methane production in humans and rats[J]. Sci Rep,2017, 7(1):7329.
|
| [8] |
Ye Z, Chen O, Zhang R, et al. Methane attenuates hepatic ischemia/reperfusion injury in rats through antiapoptotic, anti-inflammatory, and antioxidative actions[J]. Shock, 2015, 44(2): 181-187.
|
| [9] |
Meng Y, Jiang Z, LiN,et al. Protective effects of methane-rich saline on renal ischemic-reperfusion injury in a mouse model[J]. Med Sci Monitor, 2018, 24: 7794-7801.
|
| [10] |
Chen O, Ye Z, Cao Z, et al.Methane attenuates myocardial ischemia injury in rats through anti-oxidative, anti-apoptotic and anti-inflammatory actions[J].Free Radic Biol Med,2016,90: 1-11.
|
| [11] |
Liu L, Sun Q, Wang R, et al. Methane attenuates retinal ischemia/reperfusion injury via anti-oxidative and anti-apoptotic pathways[J]. Brain Res, 2016, 1646: 327-333.
|
| [12] |
Wang W, Huang X, Li J, et al. Methane suppresses microglial activation related to oxidative, inflammatory, and apoptotic injury during spinal cord injury in rats[J]. Oxid Med Cell Longev, 2017: 2190897.
|
| [13] |
Boros M, Ghyczy M, rces D, et al. The anti-inflammatory effects of methane[J]. Crit Care Med,2012, 40: 1269-1278.
|
| [14] |
Adam-Vizi V, Chinopoulos C. Bioenergetics and the formation of mitochondrial reactive oxygen species[J]. Trends Pharmacol Sci,2006, 27(12): 639-645.
|
| [15] |
Miller K W, Hammond L, Porter E G. The solubility of hydrocarbon gases in lipid bilayers[J]. Chem Phys Lipids,1977, 20: 229-241.
|
| [16] |
Meyer M, Tebbe U, Piiper J. Solubility of inert gases in dog blood and skeletal muscle[J]. Pflugers Arch,1980, 384:131-134.
|
| [17] |
Koyano F, Okatsu K, Kosako H, et al. Ubiquitin is phosphorylated by PINK1 to activate parkin[J]. Nature, 2014, 510(7503): 162-166.
|
|
|
|
2019, Vol. 30 
