膜联蛋白A1拟肽Ac2-26对大鼠心肺复苏后脑神经损伤的保护作用

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Abstract Objective To explore the protective effect of Annexin A1 peptide Ac2-26 on brain injury after cardiopulmonary resuscitation in rats. Methods Forty-five rats were randomly divided into the sham group, control group and Ac2-26 group, with 15 in each.The sham group underwent only conducted tracheal intubation and arteriovenous puncture while the control group and Ac2-26 group were subjected to five-minute asphyxia.After the recovery of spontaneous circulation, rats in the Ac2-26 group were intravenously injected with Ac2-26(1 mg/kg),but an equal amount of saline was infused in the control group. The rats were evaluated preoperatively and at 24 h, 48 h and 72 h after spontaneous circulation recovery according to the neurological deficit score. The ELISA method was used to determine the concentrations of IL-1β, IL-6 and TNF-α in the serum of each group at 72 h. After three days of spontaneous circulation recovery, the rat cortex and hippocampus were taken to measure the expression of NF-κB in each group by Western Blot. Results The survival rate was 100% in the sham group, 53.3% in the control group and 73.3% in the Ac2-26 group three days after operation. Compared with the control group, the cumulative survival rate of the Ac2-26 group was significantly increased three days after cardiopulmonary resuscitation. Compared with the sham operation group, the scores of neural function defects in the control group decreased at each time point (P<0.01).However, compared with the control group, the neurological impairment score in the Ac2-26 group increased at each time point (P<0.01).The serum levels of IL-1β[(35.9±6.2)pg/ml], IL-6[(32.9±5.7)pg/ml] and TNF-α[(25.5±3.5)pg/ml]in the Ac2-26 group three days after operation were significantly lower than those in the control group[(82.5±10.2)pg/ml,(64.5±9.3)pg/ml,(39.9±7.6)pg/ml](P<0.05). The expression level of NF-κB protein in the prefrontal cortex and hippocampus of rats in the Ac2-26 group[(0.174±0.039), (0.298±0.014)] was significantly lower than that of the control group[(0.393±0.056), (0.450±0.027)](P<0.05). There was no significant difference in the expression level of NF-κB protein between the Ac2-26 group and the sham group[(0.174±0.039) vs (0.177±0.054),(0.298±0.014) vs (0.272±0.022)]. Conclusions Annexin A1 peptide Ac2-26 has neuroprotective effects on brain injury after cardiopulmonary resuscitation in rats.

Key words： ANXA1 Ac2-26 cardiopulmonary resuscitation spontaneous circulation recovery

Received: 17 February 2020

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2020/V31/I9/757

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