人染色体端粒酶基因、TCT结合HPV检测用于宫颈组织病变的诊断价值

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Abstract Objective To explore the diagnostic value of human chromosome telomerase (hTERC) genes and liquid based thin-layer cytology (TCT) combined with human papilloma virus (HPV) detection for cervical histopathology, and to analyze the efficacy of combined detection in the diagnosis of cervical lesions. Methods One hundred and fifty patients with cervical lesions screened in Henan Armed Police Corps Hospital between September 2019 and November 2020 were selected. The pathological tissue biopsy under colposcopy was taken as the gold standard. These patients were divided into the inflammation group, CIN1 group, CIN2 group, CIN3 group and invasive cancer group. All the patients were tested for TCT, HPV and hTERC genes respectively. The sensitivity, specificity and coincidence rate of the three detection methods were analyzed. Results The positive expression rate of hTERC genes increased (24.1%,27.0%,61.9%,73.3%,75.0%) with the cytological grade(inflammation,CIN1,CIN2,CIN3, and invasive cancer), so did the positive rate of HPV (34.5%, 64.9%, 71.4%, 83.3%, 91.7%) and TCT(0,70.3%,73.8%, 80.0%, 100.0%). The sensitivity of HPV+TCT detection was 82.95%, the specificity was 82.76%, and the coincidence rate was 85.33, compared with 89.26% 86.2% and 88.67% for HPV+hTERC. Conclusions The coincidence rate of combined diagnosis is significantly higher than that of a single method. HPV+hTERC gene detection has the best results, which can effectively help identify cervical tissue lesions.

Key words： thin-layer cytology human papilloma virus human chromosome telomerase gene cervical tissue lesions

Received: 20 July 2021

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	Articles by authors
	JIN Mengjie
	HU Qian
	LI Qiang
	WU Hui

Cite this article:

JIN Mengjie,HU Qian,LI Qiang等. Diagnostic value of human chromosome telomerase gene and TCT combined with HPV detection for cervical histopathology[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2021, 32(12): 1025-1029.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2021/V32/I12/1025

[1]	牛艳昕, 王楠. hTERC基因与宫颈癌发生、发展相关性[J]. 医学研究杂志, 2018, 47(6):164-166.
[2]	廖婧, 许长俊, 孙春意, 等. hTERC基因检测与HC2,SPR技术检测HPV在宫颈病变中的诊断价值[J]. 昆明医科大学学报, 2020,41(9):23-26.
[3]	刘洋, 杨德红, 孙春意,等. 宫颈病变中C-MYC,hTERC表达及其与SPR技术检测HPV结果的相关性分析[J]. 昆明医科大学学报, 2019,40(10)85-88.
[4]	余杰, 鄢贵芹. hTERC基因,高危HPV检测联合TCT在宫颈癌筛查中的意义[J]. 中国妇幼保健, 2017, 20(32): 84-86.
[5]	江维, 曾四元, 梁美蓉,等. 宫颈液基细胞学检查与hTERC基因检测联合应用对阴道镜活检漏诊高级别上皮内瘤变的诊断效能[J]. 山东医药, 2018, 58(3): 62-64.
[6]	付海英, 张丽萍, 吴佳彬. 宫颈薄层液基细胞学联合HPV检测与宫颈组织病理学的相关性[J]. 中国妇幼保健, 2020,35(3):560-561.
[7]	加秋萍, 贺清波. 评估TCT,HPV,c-MYC,hTERC基因联合检测在宫颈癌筛查中的应用价值[J]. 中国医师杂志, 2017, 19(9): 1370-1373.
[8]	黄燕, 朱晓云, 李天英. 人乳头瘤病毒DNA检测联合液基细胞学检查在宫颈病变筛查中的意义[J]. 检验医学, 2019, 34(4): 37-41.
[9]	张威, 杜艳敏, 张欢欢. TCT,HPV-DNA及hTERC基因联合检测对提高宫颈癌及癌前病变的筛查准确性的影响[J]. 航空航天医学杂志, 2017, 28(4): 402-403.
[10]	杜芳. 分析组织病理学诊断子宫颈病变的临床应用价值[J]. 智慧健康, 2018, 4(4): 117-118.
[11]	窦娟. 高危型HPV-DNA与TCT配合阴道镜在早期宫颈病变筛查中的分析[J]. 首都食品与医药, 2018, 25(14): 23-24.
[12]	姜仙姣, 李奕平, 牟虹. TCT联合HPV分型检测在宫颈癌前病变筛查中的应用分析[J]. 浙江临床医学, 2017,19(3): 481-482.
[13]	彭智慧,张钰华,李桂民,等. TCT与HR-HPV-DNA联合检查对宫颈癌筛查效果分析[J]. 社区医学杂志, 2020, 18(14): 47-50.
[14]	宋明慧. TERC基因、HPVL1蛋白在宫颈癌筛查中的应用研究[J]. 中国生育健康杂志, 2018,29(5): 470-472.
[15]	周晓花, 宋芳, 吕讷男,等. HR-HPV阳性TCT阴性女性的宫颈上皮组织病理学结果分析[J]. 医学综述, 2020, 26(1): 183-186.
[16]	赵冰英, 胡红彦, 张丽丽. TCT联合HPV—DNA及端粒酶检测在宫颈癌前病变[J]. 健康前沿, 2018, 27(9): 174-174.
[17]	杨梅, 宁旦. 宫颈端粒酶hTERC基因的表达和宫颈高危型HPV感染的型别的相关研究[J]. 亚洲肿瘤科病例研究, 2018, 7(4): 51-55.
[18]	李月, 俞北伟, 滕贤麟,等. hTERC扩增检测联合宫颈活检对HPV-55和HPV-83致病能力的定性研究[J]. 浙江实用医学, 2017,22(5): 315-318.
[19]	李海璇, 李玲. 宫颈病变中hTERC基因表达与人乳头瘤病毒感染关系的研究[J]. 青海医药杂志, 2017,47(4): 6-9.
[20]	娜日苏, 曹亚宁, 张晓杰,等. hTERC基因在蒙古族患者宫颈病变组织中的表达及其意义[J]. 内蒙古民族大学学报(自然科学版), 2019, 34(4): 95-98.
[21]	李春琳, 张红平, 王应海,等. hTERT基因多态性与宫颈癌前病变关联性研究[J]. 中华肿瘤防治杂志, 2019,26(18): 1323-1328.
[22]	王登海. TCT联合HPV-DNA及端粒酶检测在宫颈癌前病变评估中的应用价值[J]. 检验医学与临床, 2017, 21(14):3193-3194.
[23]	LUO Gupo, ZENG Xi, CAO Hanyu, et al. The clinical significance of HPV L1/PD-L1 tests combined with colposcopy for cervical precancerous lesions and cervical cancer[J]. J Sichuan University (Med Sci Edition), 2021, 52(3):516-522.
[24]	刘东涌. 人端粒酶TERT蛋白和P16蛋白在宫颈病变中的表达研究[J]. 中外医疗, 2019, 38(13):43-45.