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| Effects of spermidine on bone microstructure of femurs of senile mice |
| SU Jingke1, SU Jinghua2, HAN Ning1, TIAN Xiaoyu3, DANG Ruijie3, FENG Jin3, GENG Yanqiu1 |
| 1. Department of Nephrology, 2. Health Medical Center, the Third Medical Center, PLA General Hospital, Beijing 100039,China; 3. Institute of Orthopedics, the First Medical Center, PLA General Hospital, Beijing 100853,China |
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Abstract Objective To investigate the effects of spermidine on bone microstructure of femurs of senile mice. Methods Twenty 21-month-old C57 mice were randomly divided into the vehicle group (n=10 ) and spermidine group (3 mmol/L spermidine in drinking water, n=10). Body weight was measured weekly during the study. After 12 weeks of intervention, biochemical indexes of bone metabolism in serum and urine were tested and histomorphometry of femurs was measured by micro-CT (microcomputed tomography). Results Compared with the vehicle mice, spermidine had no effect on the weight of senile mice. Spermidine intervention significantly increased serum levels of ALP (P＜0.05), elevated the trabecular bone volume per tissue volume (Tb.BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th)and bone mineral density (BMD), and reduced trabecular separation (Tb.Sp). Conclusions Spermidine attenuates age-associated osteoporosis in senile mice.
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Received: 10 June 2021
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2022, Vol. 33 
