人参皂苷Rg1对大鼠海水淹溺性肺损伤的保护作用及其机制

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Abstract Objective To determine the protective effects and potential mechanisms of ginsenoside Rg1 on lung injury induced by sea water drowning.Methods One hundred and twenty-eight male SD rats were randomly divided into four groups: the control group (group C), sea water+saline group (group S), sea water group+20 mg/kg Rg1(group L) and sea water group+100 mg/kg Rg1(group H). Rats were intravenously injected with different doses of Rg1 or saline before a standard model of sea water drowning was established in group S, L and H. The arterial PaO₂, PaCO₂, wet to dry weight ratio(W/D), protein concentrations of bronchoalveolar lavage fluid (BALF), MDA, SOD and MPO activity, cytokines expressions and histological changes were measured at the following time points as designated:before drowning(T₀) and 30 min(T₁), 1 h (T₂), 2 h(T₃) and 4 h(T₄) after drowning.Results PaO₂ at T₁,T₂,T₃ and T₄ in group S and L was significantly lower than in group H (P<0. 05), but PaCO₂ was significantly higher(P<0. 05).No significant difference was observed between group S and group L. The W/D ratio, BALF protein concentration, MDA and MPO activity were significantly higher in group S and L than in group H at T₃ and T₄(P<0.05). The activity of SOD in group S and L was significantly lower than in group H(P<0 05). Compared with group S, the expressions of such cytokines as TNF-α, IL-1β and IL-6 were significantly decreased. HE staining showed severe alveolar rupture, edema, and inflammatory cell infiltration in group S.Conclusions Pretreatment with Rg1 can effectively protect rats from sea water drowning induced lung injury in the early stage(within 4 hours). The underlying mechanism is possibly associated with the suppression of inflammatory responses and oxidative stress.

Key words： ginsenoside Rg1 sea water drowning lung injury oxidative stress

Received: 20 October 2021

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	TIAN Ye
	GE Shuaina
	LI Jun
	TAO Tianzhu

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TIAN Ye,GE Shuaina,LI Jun等. Protective effects of ginsenoside Rg1 on sea water drowning induced lung injury in rats[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2022, 33(2): 140-143.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2022/V33/I2/140

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