基于雅培ARCHITECT i2000的CMIA在梅毒血清抗体诊断中的应用策略

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Abstract Objective To investigate the applicability of Abbott ARCHITECT I2000 based chemiluminescent particle immunoassay (CMIA) in detecting serum syphilis antibodies. Methods Two hundred and seventy-one cases of CMIA positive serum samples (S/CO≥1.0) in syphilis screening were collected before these samples were retested using treponema pallidum particle agglutination (TPPA). The ability of CMIA to detect TPPA-positive cases was evaluated via receiver operating characteristic curve (ROC) analysis. Furthermore, these CMIA positive samples were divided into four groups according to their S/CO values: group A (1.0≤S/CO<3.0, n=85), group B (3.0≤S/CO<5.0, n=26), group C (5.0≤S/CO<7.0, n=17) and group D (S/CO≥7.0, n=143). The differences in TPPA positive rates among the four groups were analyzed. Meanwhile, a cutoff-positive rate curve was drawn to identify the optimal retest cutoff using TPPA. Results Among the 271 CMIA positive samples, 196 cases were positive for TPPA. The positive coincidence rates of TPPA retest from group A to group D were 20.00%, 76.92%, 94.12% and 100.00%, respectively, so the difference was statistically significant (W = 14253, P<0.001). When the reexamination cutoff was 5.1, the positive rate of TPPA reexamination reached 100%. Conclusions CMIA is of great value in detecting syphilis serum antibodies. It is recommended that positive CMIA results can be reported directly when S/CO value is above 5.1, and that TPPA reexamination shoud be performed when S/CO values range from 1.0 to 5.1. This strategy might be able to reduce false positive reports.

Key words： chemiluminescence particle immunoassay treponema pallidum antibody syphilis particle agglutination test chemiluminescence method for syphilis

Received: 20 October 2021

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	ZHANG Jianguang
	DU Zhenhua
	ZHANG Guifen

Cite this article:

ZHANG Jianguang,DU Zhenhua,ZHANG Guifen. Abbott ARCHITECT I2000 based CMIA in detecting serum syphilis antibodies[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2022, 33(3): 198-201.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2022/V33/I3/198

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