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| Protective effect of cannabidiol on high glucose-induced podocytes MPC5 apoptosis |
| SU Jinghua1, JIAO Ting2, HAN Ning3, SU Jingke3, DANG Ruijie4, LI Lin4, ZHANG Haisong2 |
1. Health Medical Center, 3. Department of Nephrology, the Third Medical Center of Chinese PLA General Hospital,Beijing 100039, China;
2. Oncology Division, Affiliated Hospital of Hebei University, Baoding 071000, China;
4. Medical School of Chinese PLA, Beijing 100853, China |
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Abstract Objective To investigate the effect of cannabidiol (CBD) on podocytes MPC5 apoptosis induced by high glucose.Methods MPC5 cells were randomly divided into vehicle group, high glucose (HG) group and CBD group. CCK-8 method was used to access cell proliferation, flow cytometry was used to evaluate apoptotic rate, and western blot was used to investigate protein level of Nephrin and apoptosis-related proteins Bax and Bcl-2.Results Compared with the Vehicle group, HG significantly inhibited MPC5 proliferation and promoted MPC5 apoptosis [(25.27±2.15)% vs. (8.43±0.70)%], while CBD obviously promoted MPC5 proliferation and inhibited MPC5 apoptosis [(12.83±1.05)% vs. (25.27±2.15)%; P＜0.05] in the high glucose environment. Meanwhile, CBD administration increased Nephrin expression [(0.77±0.06) vs. (0.32±0.10)], and reduced Bax/Bcl-2 [(1.02±0.09) vs. (12.30±0.09); P＜0.05].Conclusions CBD attenuates high glucose-induced MPC5 apoptosis.
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Received: 09 October 2021
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2022, Vol. 33 
