七氟醚上调PI3K/Akt/mTOR活性对小鼠心肌缺血再灌注损伤中自噬水平的影响

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Abstract Objective From the perspective of PI3K/Akt/mTOR pathway, to explore the potential effect of sevoflurane on autophagy levels in mice with myocardial ischemia-reperfusion injury. Methods Twenty-four mice were randomly divided into 4 groups (n=6): sham operation group(only chest opening without ligation), ischemia-reperfusion group (ligating the left anterior descending coronary artery as ischemia-reperfusion operation under pentobarbital anesthesia), sevoflurane group (surgery under sevoflurane anesthesia), and sevoflurane+LY294002 group (LY294002, PI3K blocker, was injected daily one week before ischemia-reperfusion surgery and the rest was the same as in Sevoflurane group). Plasma was collected to detect the levels of myocardial injury markers, creatine kinase-MB (CK-MB) and cardiac troponin I (cTnI). Myocardial tissue was detected by western blot method to detect autophagy markers, LC3 and Beclin1, and signaling pathway proteins, phosphatase 3-kinase (PI3K), protein kinase B (Akt), rapamycin (mTOR) total protein and phosphorylated protein levels. Results Compared with sham operation group, the levels of plasma CK-MB, cTnI, markers of cardiac autophagy LC3 and Beclin1 in the other three groups were significantly increased (P<0.05), and the ratios of phosphorylated PI3K, Akt and mTOR to total protein significantly decreased (P<0.05). Compared with sham operation group, the total protein levels of PI3K, Akt and mTOR in sevoflurane group were not significantly different. The levels of PI3K and Akt significantly increased (P<0.05), and the level of mTOR protein significantly decreased (P<0.05) in ischemia-reperfusion group. The level of PI3K in sevoflurane +LY294002 group significantly increased (P<0.05), and the protein levels of Akt and mTOR significantly decreased (P<0.05). Compared with ischemia-reperfusion group, plasma LEVELS of CK-MB and cTnI, LC3 and Beclin1 in sevoflurane group and sevoflurane +LY294002 group significantly decreased (P<0.05). Compared with the ischemia-reperfusion group, the ratio of phosphorylated PI3K, Akt and mTOR protein to total protein levels in sevoflurane group significantly increased (P<0.05). Compared with sevoflurane group, plasma LEVELS of CK-MB, cTnI, LC3 and Beclin1 in sevoflurane +LY294002 group significantly increased (P<0.05). The levels of phosphorylated PI3K, Akt and mTOR protein to total protein in sevoflurane +LY294002 group significantly decreased (P<0.05). Conclusions Sevoflurane inhibits the excessive autophagy induced by ischemia-reperfusion, and the possible mechanism is that it upregulates the phosphorylation level of PI3K/Akt/mTOR pathway protein.

Key words： sevoflurane ischemia-reperfusion autophagy mTOR

Received: 10 November 2021

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	Articles by authors
	REN Nihui
	GUO Zhijia
	WANG Xin
	WANG Chenggang
	ZHANG Wenjie
	TIAN Shouyuan

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REN Nihui,GUO Zhijia,WANG Xin等. Effect of sevoflurane on autophagy level in mice with myocardial ischemia-reperfusion injury[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2022, 33(5): 416-419.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2022/V33/I5/416

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