宏基因二代测序在中枢神经系统感染性疾病诊断中的应用

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Abstract Objective To investigate the application value of metagenomic next-generation sequencing (mNGS) in the diagnosis of infectious diseases of central nervous system (CNS infectious diseases).Methods The clinical data of 85 patients with CNS infectious diseases admitted to the Neurology Department of Shanxi Bethune Hospital from January 2019 to December 2021 were retrospectively analyzed to explore the advantages of mNGS.Results Twenty-one kinds of pathogens were detected by mNGS, 57 cases were positive, 48 cases were in accordance with clinical diagnosis, 9 cases were not, and the accuracy was 56.5%(48/85); 23 cases were positive by traditional methods, 22 cases were in accordance with clinical diagnosis, 1 case was not, and the accuracy was 25.9%(22/85). The difference between the two methods was significant (P<0.05).Conclusions The detection accuracy of mNGS is higher than that of the traditional method, and mNGS can be used as a complementary method to the traditional detection one, but because of the results of mNGS are inconsistent with the clinical diagnosis in many cases, the interpretation of detection results should be carefully considered in combination with clinical practice.

Key words： metagenomic next-generation sequencing central nervous system infections accuracy

Received: 29 January 2022

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	Articles by authors
	SHANG GUAN Lijuan
	WANG Hailong
	SHANG Jing
	WANG Zhijun
	HOU Miaomiao
	XUE Lanping

Cite this article:

SHANG GUAN Lijuan,WANG Hailong,SHANG Jing等. Application of metagenomic next-generation sequencing in diagnosis of infectious diseases of central nervous system[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2022, 33(6): 472-475.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2022/V33/I6/472

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