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| Role of ZFP580 in acute pancreatitis induced by caerulein through endoplasmic reticulum stress pathway |
| ZI Li1,2, LIU Guanglin1,2, ZHANG Wencheng1,2, XIA Shihaii1,2, XU Wei1,2 |
1. Department of Gastroenterology, Characteristic Medical Center of Chinese People’s Armed Police Force, Tianjin 300162, China;
2. Tianjin Key Laboratory of Hepatopancreatic Fibrosis and Molecular Diagnosis & Treatment, Characteristic Medical Center of Chinese People’s Armed Police Force, Tianjin 300162, China |
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Abstract Objective To investigate the role of zinc finger protein 580 (ZFP580) in acute pancreatitis (AP) induced by caerulein through endoplasmic reticulum (ER) stress pathway, and provide a new therapeutic target for AP treatment.Methods MTT method was used to detect cell viability. RT-PCR and Western Blot were used to detect endoplasmic reticulum stress-related mRNA and the protein expression of ZFP580 in AP model. IRE1 inhibitor (MKC-3946) was used to observe the position of ZFP580 in endoplasmic reticulum stress related signaling pathway. After Silencing ZFP580, Hochest33342 staining and WB, RT-PCT method were used to investigate the effect of ZFP580 on the apoptosis-necrosis process.Results After using cerulein to stimulate acute pancreatitis in AR42J cells, the endoplasmic reticulum stress occurred. 8 hours later, the expression of ZFP580 started to increase (showing a time-dependent increase). After using IRE1 inhibitor (MKC-3946), the expression of ZFP580 was significantly lower than that of the group without MKC-3946 treatment (P<0.05). After silencing ZFP580, the expression of XBP-1s significantly decreased. At the same time, RT-PCR and Western blot results showed that the expression of Chop in the si-ZFP580 group was significantly higher than that in the normal AP group, while the expression of Caspase-3 was lower than that in the normal AP group. The result of Hochest staining revealed that the proportion of necrotic cells in the si-ZFP580 group was significantly higher than that in the normal AP group.Conclusions ZFP580 which may be located between IRE1 and XBP-1s may play a protective role in regulating the severity of AP by improving the apoptosis-necrosis ratio.
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Received: 23 June 2021
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2022, Vol. 33 
