大麻二酚缓解高脂饮食老龄小鼠肝脏脂肪变性的实验研究

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Abstract Objective To explore the therapeutic effect and signaling mechanisms of cannabidiol (CBD) intervention on hepatic steatosis in aged mice fed with a high fat diet.Methods Thirty aged male C57BL/6J mice were randomly divided into three groups: normal diet group (NC), high-fat diet group (HFD) and cannabidiol intervention group (CBD). After intervention for 12 weeks, the serum levels of glucose,triglyceride (TG), total cholesterol (TC), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined by automatic biochemical analyzer, serum insulin level was detected by ELISA kit, and free fatty acid (FFA) level was determined by colorimetric analysis kit. Intraperitoneal glucose tolerance tests (IPGTT) and insulin tolerance test (ITT) were used to examine the glucose disposal ability. Lipid deposition in liver was detected by oil red O staining. The mRNA expression levels of lipid metabolism-related genes were evaluated by qRT-PCR. Western blot was used to detect the protein levels of AMPK and ACC.Results Compared with NC mice, the levels of blood glucose, TC, TG, FFA, ALT and AST in HFD mice significantly increased, and liver lipid deposition also increased (P<0.05). However, CBD intervention reduced the levels of parameters related to glucolipid metabolism (P<0.05), improved the ability of glucose disposal ability, alleviated lipid deposition in liver, and improved mRNA expression levels of lipid metabolism-related genes (P<0.05). Meanwhile, CBD significantly increased the protein expression levels of p-AMPK and p-ACC (P<0.05).Conclusion CBD alleviates hepatic steatosis in aged mice fed with a high fat diet, and AMPK/ACC signaling pathway may be involved in this progress.

Key words： cannabidiol hepatic steatosis AMPK/ACC signaling pathway

Received: 12 January 2022

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	Articles by authors
	XIA Wenxiu
	WU Fangli
	HOU Ying
	ZHANG Guangwei
	DANG Ruijie
	LI Lin

Cite this article:

XIA Wenxiu,WU Fangli,HOU Ying等. Research of cannabidiol intervention on hepatic steatosis in aged mice fed with a high fat diet[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2022, 33(7): 564-568.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2022/V33/I7/564

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