宫颈癌前病变PAX1基因DNA甲基化检测与HPV-DNA分型检测的效果比较

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Abstract Objective To analyze the characteristics and potential correlation between PAX1 gene methylation and HPV-DNA typing in the diagnosis and treatment of cervical precancerous lesions. Methods Patients who underwent liquid-based thin-layer cell testing (TCT) and human papillomavirus DNA (HPV-DNA) typing in the Department of Obstetrics and Gynecology of the Third Medical Center of PLA General Hospital from June 2021 to March 2022 were selected. A total of 194 patients with abnormal TCT results≥ atypical squamous cells of unknown significance (ASCUS) and/or HR-HPV positive and 130 patients with abnormal cervical tissue screening results who were referred for colposcopic biopsy were included in this study. TCT, HPV-DNA typing and quantitative PAX1 gene methylation were performed in all patients. Results Compared with CIN3+ (CIN3 and cervical cancer) and normal/cervicitis, CIN1 and CIN2, the methylation value of PAX1 gene in CIN3+ patients was significantly higher than that in normal, CIN1 and CIN2 patients. The methylation values of PAX1 gene from high to low were 18.21 (17.56, 18.66), 20.5 (20.07, 20.80), 20.41 (20.19, 20.72), 20.21 (19.77, 20,42), respectively. The TCT results showed that there were 45 patients with ASCUS, CIN2+ (CIN2, CIN3 and cervical cancer) compared with normal/CIN1, and the methylation value of PAX1 gene CIN2+ was significantly higher than that of normal/CIN1. The methylation values of PAX1 gene from high to low were 18.87 (17.80, 20.26) and 20.51 (20.19, 20.73), respectively. The methylation values of PAX1 gene in HPV16 and 18 positive patients were significantly higher than those in non-HPV16/18 positive patients. The methylation values of PAX1 gene in HPV16 positive and high-risk non-HPV16/18 positive patients were 19.10 (17.97, 19.88) and 20.33 (20.00, 20.50), respectively. The methylation levels of PAX1 gene in HPV18-positive and non-HPV16/18 positive patients were 19.49 (18.65, 20.31) and 20.33 (20.00, 20.50), respectively. However, there was no significant difference in the methylation value of PAX1 gene between single HPV16 positive patients and single HPV18 positive patients). Conclusions The methylation of PAX1 gene is related with the progression of cervical lesions and the type of HPV infection. The methylation values of PAX1 gene in patients with single HPV16 positive and single HPV18 positive are significantly higher than those in patients without HPV16/18 positive. In ASCUS patients, the methylation value of PAX1 gene in CIN2+ is significantly higher than that in normal /CIN1. It is suggested that PAX1 gene methylation detection is helpful for early identification of CIN3+ and ASCUS cervical high-grade lesions, and also helps to reduce the colposcopy referral rate of ASCUS patients.

Key words： PAX1 methylation detection human papillomavirus DNA cervical lesions

Received: 11 July 2022

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R711.74

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	LI Jialu
	DONG Yuan
	WANG Yu
	ZHNAG Xiaoli

Cite this article:

LI Jialu,DONG Yuan,WANG Yu等. Comparison of PAX1 gene DNA methylation detection and HPV-DNA typing in diagnosis and treatment of cervical precancerous lesions[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2023, 34(1): 18-21.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2023/V34/I1/18

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