尿石素A对高脂饮食老龄小鼠白色脂肪棕色化的影响

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Abstract Objective To investigate the effects of urolithin A on browning of white adipose in aged mice fed on a high-fat diet. Methods Eighteen aged mice were randomly divided into control group, high-fat diet (HFD) group and urolithin A administration group (UA), with 6 mice in each group. UA group was given urolithin A (Tween-80 aid solution), control group and HFD group were given the same dose of Tween-80. After the experiment, the body weight and heat production were detected, the morphology of adipocytes was observed by HE staining. qRT-PCR and Western blot were used to investigate mRNA and protein levels of uncoupling protein 1 (UCP1), peroxisome proliferator-activated receptor γ (PPARγ) and peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α). Results Compared with the control group, the body weight of HFD group significantly increased, the heat production of the daytime[(0.45±0.03) kcal/h vs. (0.62±0.05) kcal/h] and nighttime[(0.49±0.05) kcal/h vs. (0.73±0.06) kcal/h] significantly decreased(P<0.05), and the cell size of white adipose tissue increased. Moreover, UCP1, PPARγ and PGC-1α expressions decreased (P<0.05). However, urolithin A intervention reduced the body weight of high-fat diet mice, increased daytime heat production [(0.57±0.03) kcal/h vs. (0.45±0.03) kcal/h] and nighttime heat production [(0.69±0.07) kcal/h vs. (0.49±0.05) kcal/h], and promoted the transcriptional expression of UCP1, PPARγ and PGC-1α expressions (P<0.05). Conclusions Urolithin A can promote the browning of white adipose tissue in aged mice fed on a high-fat diet.

Key words： urolithin A obesity white adipose tissue brown adipose tissue

Received: 20 December 2022

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	LIU Xingyu
	LI Hui
	LI Jing
	WANG Ying
	ZHAO Lisheng
	FENG Chunzhen

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LIU Xingyu,LI Hui,LI Jing等. Effects of urolithin A on browning of white adipose in aged mice fed on a high-fat diet[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2023, 34(11): 926-929.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2023/V34/I11/926

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