红景天苷对血管生成及骨血管偶联的影响

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Abstract Objective To study the effect of salidroside (SAL) on angiogenesis and bone vessel coupling. Methods MTS method was used to investigate the effect of osteoblast-conditioned medium on the proliferation activity of vascular endothelial cells. Transwell cell migration assay was used to investigate the effect of osteoblast-conditioned medium on the migration of vascular endothelial cells. Matrigel lumen formation experiment was used to investigate the effect of osteoblast-conditioned medium on lumen formation length. Fetal metatarsal angiogenesis assay was used to evaluate the effect of SAL on the sprouting area of endothelial cells. Results Compared with the conditioned culture medium group, the cell activity of the conditioned culture medium group after SAL treatment significantly increased, and the differences were statistically significant (P<0.01). The number of vascular endothelial cell migration and the length of HUVEC lumen formation in SAL group significantly increased compared with that in conditioned culture medium group, and the difference was statistically significant (P<0.01). The area of endothelial cell budding in SAl and vascular endothelial growth factor (VEGF) treatment groups was significantly larger than that in the control group, with statistical significance (P<0.01), and the above effects could be blocked by the anti-VEGF antibody Avastin. Conclusions SAL is a good angiogenic drug monomer, and can promote bone vascular coupling by up-regulating the expression of VEGF.

Key words： salidroside vascular endothelial cell vascular endothelial growth factor angiogenesis bone vessel coupling

Received: 10 June 2023

ZTFLH:	R965.1
	R967.3

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	Articles by authors
	QI Lin
	HAN Xinyu
	QI Lu
	WANG Yue
	ZHANG Lei

Cite this article:

QI Lin,HAN Xinyu,QI Lu等. Effect of salidroside on angiogenesis and bone vessel coupling[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2024, 35(1): 1-5.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2024/V35/I1/1

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