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| Value of cuproptosis gene LIPT1 in diagnosis and prognosis of brain gliomas |
| LIN Yanchen1, XU Wei2, YING Cao3, LIU Rui3, ZHANG Yuyu1, DAI Erqing1, LI Jingjing4 |
1. Department of Rehabilitation Medicine, 2. Department of Pathology, Characteristics Medical Center of Chinese People's Armed Police Force, Tianjin 300162, China;
3. Graduate School of Tianjin University of Traditional Chinese Medicine, Tianjin 314000, China;
4. Department of Pharmacy, the Fourth Central Hospital of Tianjin, Tianjin 300140, China |
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Abstract Objective To investigate the potential value of the cuproptosis gene LIPT1 in the diagnosis and prognosis of glioma. Methods The expression of 10 cuproptosis genes in glioma was mined using TCGA database. The expression of LIPT1 in 18 glioma patients was detected by IHC and qRT-PCR. The expression of LIPT1 in HEB, U87 and U251 cells was detected by qRT-PCR. ROC curve was used to predict the diagnostic efficacy of LIPT1 in glioma. Kaplan-Meier survival curve and Cox analysis were used to explore the prognostic value of LIPT1. ssGSEA database was used to evaluate the effect of LIPT1 on immune invasion of glioma. To explore the relationship between LIPT1 and immunological checkpoints, DNA methylation site and DNA mismatch repair genes in gliomas, fFunctional enrichment of LIPT1-associated genes was performed with GO, KEGG, and GSEA. Animal TFDB3 predicts LIPT1-associated transcription factors. The GSCA database was utilized to predict potential drugs targeting LIPT1 and hub genes. Results LIPT1 expression in glioma was higher than in normal tissues (3.03 vs. 2.28, P<0.05). The immune scores of grade Ⅱ, Ⅲ and Ⅳ gliomas were different [(1.47±0.51) vs. (4.94±1.25) vs. (9.88±1.65), P<0.05]. The mRNA levels of LIPT1 in HEB, U87 and U251 cells were different [(0.22±0.03) vs. (0.45±0.04) vs. (0.58±0.05), P<0.05]. The AUC value of LIPT1 in glioma diagnosis was 0.87. The OS, DSS, and PFI in the high LIPT1 expression group were shorter than those in the low LIPT1 expression group (P<0.05). The LIPT1 expression was correlated with immune infiltration changes and the expression of immune checkpoint, DNA methylation site and DNA mismatch repair gene (P<0.05). Transcription factors such as FOXG1 were predicted to possibly bind to LIPT1. Drugs such as docetaxel might treat gliomas by targeting and regulating LIPT1-related hub genes. Conclusions LIPT1 may be a potential biomarker and may be associated with poor prognosis of glioma.
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Received: 15 November 2023
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2024, Vol. 35 
