妊娠期高血压患者胎盘组织中circRNA 表达差异及其潜在作用机制

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Abstract Objective To detect the difference of circular RNA (circRNA) expression in placentas between patients with gestational hypertension and healthy women based on high-throughput sequencing, and to explore the pathogenesis of gestational hypertension. Methods Six pregnant women with gestational hypertension admitted to the First Medical Center of PLA General Hospital from February 2018 to December 2018 were selected as the research objects (group M), and six healthy pregnant women in the same period were selected as the control group (group C). Placental tissues from patients in group M and group C were sequenced after quality control using BWA-MEM, circRNAs were identified using CIRI2, and the source of circRNAs was determined. CircRNA differential expression analysis was performed using DEGseq. The target miRNA of circRNA was predicted by miRanda software and miRBase sequence of this species, and the corresponding mRNA was predicted by Targetscan database. GO and KEGG enrichment analysis were performed on the differentially expressed circRNA target genes, and the genes were imported into String online database for cluster analysis, and the genes at the core of the network were searched. Results There were 31 differential circRNAs in the two groups of samples, 14 down-regulated and 17 up-regulated, among which the first five up-regulated circRNAs were circRNA00809, circRNA00593, circRNA00166, circRNA00184 and circRNA00028 respectively, and the first five down-regulated circRNAs were circRNA00520, circRNA00714, circRNA02491, circRNA00841 and circRNA01454 respectively. GO enrichment analysis showed that the main biological process of enrichment was reproduction process. The main enriched cell component was extracellular region. The main enriched molecular function was electron transfer activity. Metabolism was the most involved in the enrichment of KEGG pathway. Interaction network analysis showed that NOTCH1/2 was at the core. Conclusions The differential expression of 31 circRNAs in placenta is associated with gestational hypertension, which may play a role by regulating metabolic pathways, and NOTCH1/2 and other genes are at the center of regulatory network.

Key words： gestational hypertension high-throughput sequencing circRNA placenta

Received: 17 July 2023

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	LV Jungang
	ZHAI Li
	LIU Jinlong
	ZHANG Lin
	WANG Na
	PANG Aibo
	YAN Zhifeng

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LV Jungang,ZHAI Li,LIU Jinlong等. Differential expression of circRNA in placenta of patients with gestational hypertension and its potential mechanism[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2024, 35(4): 329-334.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2024/V35/I4/329

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